The Brain Atlas (www.proteinatlas.org/humanproteome/brain) provides the protein expression of the mammalian brain by visualization and integration of data from three mammalian species (human, pig, and mouse). Transcriptomics data is combined with affinity-based protein in situ localization down to single-cell detail. The expression data is based on human protein-coding genes and one-to-one orthologues in pig and mouse. The gene expression in the brain is visually summarized into 10 main brain regions (olfactory region, cerebral cortex, amygdala, hippocampal formation, basal ganglia, hypothalamus, thalamus, midbrain, combined pons and medulla, and cerebellum) that are used for regional expression abundance classification. In the Brain Atlas, expression information is also shown for the spinal cord, corpus callosum, retina, and pituitary gland. Each of the 10 brain regions can be reviewed and further explored on individual pages, which provide classification overviews, interactive lists and figures, and highlighted examples of regionally specialized cells and protein expression. High-resolution, stained images derived from antibody-based immunolabeling techniques are also included in the atlas.

Key publication

• Sjöstedt E et al., An atlas of the protein-coding genes in the human, pig, and mouse brain. Science. (2020)
  PubMed: 32139519 DOI: 10.1126/science.aay5947

Other selected publications

• Sjöstedt E et al., Defining the Human Brain Proteome Using Transcriptomics and Antibody-Based Profiling with a Focus on the Cerebral Cortex. PLoS One. (2015)
  PubMed: 26076492 DOI: 10.1371/journal.pone.0130028

• Mulder J et al., Tissue profiling of the mammalian central nervous system using human antibody-based proteomics. Mol Cell Proteomics. (2009)
  PubMed: 19351664 DOI: 10.1074/mcp.M800539-MCP200

• Mulder J et al., Systematically generated antibodies against human gene products: high throughput screening on sections from the rat nervous system. Neuroscience. (2007)
  PubMed: 17478047 DOI: 10.1016/j.neuroscience.2007.02.054