CELL LINES

Name

Description of cell line

Original reference

Origin

Sex & Age 

Source

Cellosaurus (ExPaSy)

Myeloid

HEL Erythroleukemia cell line (AML M6 in relapse after treatment for Hodgkin's disease) Martin et al., Science 216: 1233 (1982) Peripheral blood Male, 30 DSMZ CVCL_0001
HL-60 Acute promyelocytic leukemia (APL) cell line Collins et al., Nature 270: 347-349 (1977) Peripheral blood Female, 35 DSMZ CVCL_0002
HMC-1 Mastcell leukemia cell line Butterfield et al., Leukemia Res. 12: 345-355 (1988) Peripheral blood Male, 52 KN1 CVCL_0003
K-562 Chronic myeloid leukemia (CML) cell line Lozzio et al., J Natl Cancer Inst 50: 535 (1973) Pleural effusion Female, 53 DSMZ CVCL_0004
NB-4 Acute promyelocytic leukemia (APL) cell line Lanotte et al., Blood 77: 1080 (1991) Bone marrow Female, 23 DSMZ CVCL_0005
THP-1 Acute monocytic leukemia (AML) cell line Tsuchiya et al., Int J Cancer 26: 171 (1980) Peripheral blood Male, 1 DSMZ CVCL_0006
U-937 Myelomonocytic histiocytic lymphoma cell line Sundström C, Nilsson K., Int J Cancer 17:565-577 (1976) Pleural effusion Male, 37 KN1 CVCL_0007

Lymphoid

Daudi Human Burkitt lymphoma cell line Klein et al., Cancer Res 28: 1300 (1968) Orbital biopsy Male, 16 DSMZ CVCL_0008
HDLM-2 Hodgkin lymphoma cell line Drexler et al., Leuk Res 10: 487 (1986) Pleural effusion Male, 74 DSMZ CVCL_0009
Karpas-707 Multiple myeloma cell line Karpas et al., Lancet Apr 24;1(8278): 931-3 (1982) Bone marrow and peripheral blood Male, 53 KN1 CVCL_0010
LP-1 Multiple myeloma cell line Pegoraro et al., Blood 73: 1020 (1989) Peripheral blood Female, 56 DSMZ CVCL_0012
MOLT-4 Acute lymphoblastic leukemia (T-ALL) cell line Minowada et al., J Natl Cancer Inst 49: 891 (1972) Peripheral blood Male, 19 DSMZ CVCL_0013
REH* Pre-B cell leukemia cell line (ALL, first relapse) Rosenfeld et al., Nature 267: 841–3 (1977) Peripheral blood Female, 15 DSMZ CVCL_1650
RPMI-8226 Multiple myeloma cell line Matsuoka et al., Proc Soc Exp Biol Med 125: 1246 (1967) Peripheral blood Male, 61 DSMZ CVCL_0014
U-266/70 Multiple myeloma cell line (1970, IL-6-dependent) Nilsson et al Clin Exp Immunol 7:477-489 (1970) Peripheral blood Male, 53 KN1 CVCL_0015
U-266/84 Multiple myeloma cell line (1984, in vitro differentiated) Hellman et al., Eur J Immunol 18:905-910 (1988) Peripheral blood Male, 53 KN1 CVCL_0016
U-698 B-cell lymphoma cell line (lymphoblastic lymphosarcoma) Nilsson et al., Int J Cancer 13: 808-823 (1974) Tonsil Male, 7 DSMZ CVCL_0017

Brain

SH-SY5Y Metastatic neuroblastoma, clonal subline of neuroepithelioma cell line SK-N-SH Biedler et al., Cancer Res 33: 2643 (1973) Bone marrow Female, 4 DSMZ CVCL_0019
U-138 MG Glioblastoma cell line Ponten et al., Acta Pathol Microbiol Scand 74: 465 (1968) Brain Male, 47 DSMZ CVCL_0020
U-251 MG Glioblastoma cell line Westermark B., Int J Cancer12: 438 (1973) Brain Male BW2 CVCL_0021
U-87 MG** Glioblastoma, astrocytoma cell line Ponten J., Macintyre EH., Acta Pathol. Microbiol. Scand. 74: 465-486 (1968) Brain Male, 44 LGC/ATCC CVCL_0022

Lung

A549 Lung carcinoma cell line Giard et al., J Natl Cancer Inst 51: 1417 (1973) Lung Male, 58 DSMZ CVCL_0023
SCLC-21H Small cell lung carcinoma cell line Bepler et al., J Cancer Res Clin Oncol 113: 31 (1987) Pleural effusion Male, 46 DSMZ CVCL_0024

Abdominal

CACO-2 Colon adenocarcinoma cell line Fogh J., et al. J. Natl. Cancer Inst. 58: 209 (1977) Colon Male, 72 DSMZ CVCL_0025
CAPAN-2 Pancreas adenocarcinoma cell line Kyriazis et al., Cancer Res 46: 5810 (1986) Pancreas Male, 56 DSMZ CVCL_0026
Hep G2 Hepatocellular carcinoma cell line Aden et al., Nature 282: 615 (1979) Liver Male, 15 DSMZ CVCL_0027

Breast, female reproductive system

AN3-CA Metastatic endometrial adenocarcinoma cell line Dawe et al., J Natl Cancer Inst 33: 441 (1964) Lymph node Female, 55 DSMZ CVCL_0028
EFO-21 Metastatic ovarian serous cystadenocarcinoma cell line Simon et al., J Natl Cancer Inst 70: 839 (1983) Ascites Female, 56 DSMZ CVCL_0029
HeLa Cervical epithelial adenocarcinoma cell line Scherer et al., J Exp Med 97: 695 (1953) Cervix Female, 31 DSMZ CVCL_0030
MCF7 Metastatic breast adenocarcinoma cell line Soule et al., J Natl Cancer Inst 51: 1409 (1973) Pleural effusion Female, 69 DSMZ CVCL_0031
SiHa Cervical squamous cell carcinoma cell line, integrated 1-2 copies of HPV16 Friedl F. et al.Proc., Soc. Exp. Biol. Med. 135: 543-545 (1970) Cervix Female, 55 LGC/ATCC CVCL_0032
SK-BR-3 Metastatic breast adenocarcinoma cell line Fogh J, editor. Human tumor cells in vitro. New York: Plenum Press (1975) Pleural effusion Female, 43 LGC/ATCC CVCL_0033

Urinary, male reproductive system

NTERA-2 Metastatic embryonal carcinoma cell line, cloned from TERA-2 Fogh & Trempe, Human Tumor Cells in Vitro : 115 (1975) Male, 22 DSMZ CVCL_0034
PC-3 Metastatic poorly differentiated prostate adenocarcinoma cell line Kaighn et al., Invest Urol 17: 16-23 (1979) Bone marrow Male, 62 DSMZ CVCL_0035
RT4 Urinary bladder transitional cell carcinoma cell line Rigby et al., Br J Cancer 24: 746 (1970) Urinary bladder Male, 63 DSMZ/ECACC CVCL_0036

Skin

A-431 Epidermoid carcinoma cell line Giard et al., J Natl Cancer Inst 51: 1417 (1973) Skin Female, 85 DSMZ CVCL_0037
HaCaT Keratinocyte cell line Boukamp et al., J Cell Biol Mar 106(3): 761 (1988) Skin Male NEF3 CVCL_0038
SK-MEL-30 Metastatic malignant melanoma cell line Carey et al., Proc Natl Acad Sci USA 73: 3278 (1976) Subcutis Male, 67 DSMZ CVCL_0039
WM-115 Malignant melanoma cell line Westermark B. et al, Proc Natl Acad Sci U S A. Oct; 83(19): 7197 (1986) Skin Female, 58 BW2 CVCL_0040

Sarcoma

RH-30 Metastatic rhabdomyosarcoma cell line Douglass et al., Cytogenet Cell Genet 45: 148-155 (1987) Bone marrow Male, 17 DSMZ CVCL_0041
U-2 OS Osteosarcoma cell line Ponten J., Saksela E. Int. J. Cancer 2: 434 (1967) Bone Female, 15 LGC/ATCC CVCL_0042
U-2197 Malignant fibrous histiocytoma cell line Genberg et al., Am J Pathol 135: 1185 (1989) Subcutis Male, 79 DSMZ CVCL_0043

Miscellaneous

BEWO Metastatic choriocarcinoma cell line Pattillo et al., Cancer Res 28: 1231-1236 (1968) Brain Male DSMZ CVCL_0044
HEK 293 Embryonal kidney cell line, transformed by adenovirus type 5 Graham et al., J Gen Virol 36: 59 (1977) Embryonal kidney Female DSMZ CVCL_0045
HTh 83 Anaplastic thyroid carcinoma cell line Dahlman et al., J. Pathol. 191: 376-386, 2000 Thyroid Male, 67 NEH4 CVCL_0046
TIME Telomerase-immortalized human microvascular endothelial cells (pooled) Venetsanakos et el., Exp Cell Res., Feb 1;273(1): 21-33 (2002) Skin Male LCW5 CVCL_0047
BJ Adherent fibroblast cell line Bodnar AG., et al. Science 279: 349-352 (1998) Foreskin Male LGC/ATCC CVCL_3653

Mouse

NIH 3T3 Mouse embryonal fibroblast cell line Aaronson et al., J Cell Physio 72(2): 141-148 (1968) Embryo DSMZ CVCL_0594

Notes

*
REH was previously listed as KM3. Due to misidentification, KM3 has been deleted from the DSMZ catalogue and the true identity is REH. DSMZ - False leukemia and lymphoma cell lines. Drexler et al., False leukemia-lymphoma cell lines: An update on over 500 cell lines. Leukemia 17: 416-426 (2003)
**
U-87 MG under investigation.

Source information

  1. Prof. Kenneth Nilsson, Uppsala University, Uppsala, Sweden
  2. Prof. Bengt Westermark, Uppsala University, Uppsala, Sweden
  3. Prof. Norbert E Fusenig, DKFZ, Heidelberg, Germany
  4. Assoc. Prof. Nis-Erik Heldin, Uppsala University, Uppsala, Sweden
  5. Prof. Martin McMahon, UCSF Comprehensive Cancer Center, San Fransisco, USA

Patient cell samples

Name

Description

Samples

Cells

PBMC Peripheral Blood Mononuclear Cells from healthy blood donors 15

Leukemia cells

Leukemia, AML AML 4
Leukemia, B-ALL B-ALL 3
Leukemia, B-CLL B-CLL 8
Leukemia, CML CML 5
Leukemia, T-ALL T-ALL 2

Hematopoietic cell lines

Schematic description of hematopoietic differentiation lineages, and cellular origin for hematopoietic cell lines.