Brain tissue expression of PFN2 in pons

PONS - Expression summary

Protein expressionⁱ

No data

RNA expressionⁱ

HPA Brain:	213.9	nTPM
FANTOM5:	278.2	Scaled Tags Per Million

Macaque:	503.2	nTPM
Mouse:	150.9	nTPM
Rat:	283.3	nTPM
Pig:	388.9	nTPM

PONS - SINGLE NUCLEI RNA EXPRESSIONⁱ RNA expression in the single nuclei cluster types identified in this brain region visualized by a UMAP plot (top) and a bar chart (to the right). - UMAP PLOT visualizes the cells in each cluster; where each dot corresponds to a cell. For each individual cell, mouseover reveals read count and which cluster the cell belongs to. Hovering over a cluster name will highlight the corresponding cluster in the bar chart two the right. There are 2 options for color schemes: 1) cell type color, which is based on cell type groups used in the Single cell type part of the Single cell resource and 2) cluster color, which assigns a unique color to each cluster. There are 3 options for cell intensities: 1) Intensity, which color the individual cells according to % of max (log2(read_count+1)/log2(max(read_count)+1)100) in five different bins (<1%,<25%,<50%,<75%,≥75%) 2) Interval, which color the individual cells according to fixed interval of read count (0,1,2-4,5-9,>10) 3) Hexagon, which color the hexagon on average read count of all cells inside the hexagon - The BAR CHART* shows RNA expression (nTPM) in each cluster type. Hovering over the cluster name reveals nTPM value and number of included cells. Hovering over a bar highlights the corresponding cluster in the UMAP plot above. Color-coding can be toggled on the top of the page, between 2 options: 1) cell type color, which is based on cell type groups used in the Single cell type part of the Single cell resource and 2) cluster color, which assigns a unique color to each cluster.

SINGLE NUCLEI BRAIN - PONS, NEURONSⁱ

Cell type color

Cluster color

Colored according to cell type group

Read count filter: 0

Cluster		Cell type
	c-1	Excitatory (VGLUT1/2)
	c-2	Excitatory (VGLUT1) ETV1+ Z...
	c-3	Excitatory (VGLUT1/2)
	c-4	Excitatory (VGLUT1) ETV1+ Z...
	c-5	Excitatory (VGLUT1/2)
	c-6	Excitatory (VGLUT1/2)
	c-7	Excitatory (VGLUT1/2)
	c-8	Inhibitory (GAD1/2) SLC6A5+
	c-9	Excitatory (VGLUT2)
	c-10	Excitatory (VGLUT1/2)
	c-11	Excitatory (VGLUT1/2)
	c-12	Unassigned
	c-13	Inhibitory (GAD1/2) RORB+ K
	c-14	Inhibitory (GAD1/2) SST+
	c-15	Excitatory (VGLUT1) ETV1+ Z...
	c-16	Excitatory (VGLUT2)
	c-17	Excitatory (VGLUT1/2)
	c-18	Inhibitory (GAD1/2)
	c-19	Inhibitory (GAD1) PENK+ SST
	c-20	Excitatory (VGLUT2) FOXP2+
	c-21	Excitatory (VGLUT2) LMX1A+
	c-22	Excitatory (VGLUT2) MEIS2+
	c-23	Inhibitory (GAD1/2) SLC6A5+...
	c-24	Inhibitory (GAD1/2) SLC6A5+...
	c-25	Serotonergic SLC6A4+ SLC18A...
	c-26	Inhibitory (GAD1/2) PVALB+ ...
	c-27	Excitatory (VGLUT2)
	c-28	(VGLUT3) PENK+
	c-29	MEIS2+
	c-30	LMX1A+
	c-31	Inhibitory (GAD1/2) TAC1+
	c-32	Excitatory (VGLUT2) PVALB+
	c-33	Inhibitory (GAD1/2) PVALB+ ...
	c-34	Inhibitory (GAD1/2)
	c-35	Excitatory (VGLUT2) SST+ ME...
	c-36	LC Noradrenergic SLC6A2+ SL...
	c-37	Excitatory (VGLUT2) CRH+ FO...
	c-38	Inhibitory (GAD1/2) PVALB+ ...
	c-39	Excitatory (VGLUT2)
	c-40	Excitatory (VGLUT2) PVALB+
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This data was generated by Siletti K et al. (2023), re-processed by HPA (see methods) and simplified for global overview

SINGLE NUCLEI BRAIN - PONS, NON-NEURONALⁱ

Cell type color

Cluster color

Colored according to cell type group

Read count filter: 0

Cluster		Cell type
	c-1	Oligodendrocytes
	c-2	Oligodendrocytes
	c-3	Oligodendrocyte progenitor ...
	c-4	Oligodendrocytes
	c-5	Oligodendrocytes
	c-6	Oligodendrocytes
	c-7	Microglia
	c-8	Astrocytes
	c-9	Oligodendrocytes
	c-10	Astrocytes (CD44+)
	c-11	Oligodendrocytes
	c-12	Fibroblasts
	c-13	Vascular endothelial cells
	c-14	Pericytes
	c-15	Ependymal cells
	c-16	Lymphocytes
	c-17	Macrophages
	c-18	Microglia (SPP1+ HIF1A+)
	c-19	Bergmann glia
	c-20	Immature oligodendrocytes
	c-21	Oligodendrocyte progenitor ...
	c-22	Vascular smooth muscle cell
	c-23	Monocytes
	c-24	Immature oligodendrocytes
	c-25	Fibroblasts (BNC2+ KCNMA1+ ...
	c-26	Choroid plexus epithelial c...
	c-27	Macrophage Microglia double
	c-28	OPC & lymphocytes doublets
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This data was generated by Siletti K et al. (2023), re-processed by HPA (see methods) and simplified for global overview

PONS - HPA Brain Stereo-seq

PONS - HPA Brain RNA-seq

Max subtype nTPMⁱ Max subtype nTPM is the value for the subtype with the largest average nTPM across the subtype samples.	213.9

Sample id

Sample description

nTPM

Anterior cochlear nucleus, ventral
nTPM: 142.4

2301	Female, age 85, both hemispheres	161.3
2188	Female, age 91, both hemispheres	149.6
1984	Male, age 76, both hemispheres	138.4
2090	Male, age 80, both hemispheres	120.3

Dorsal cochlear nucleus
nTPM: 136.9

2187	Female, age 91, both hemispheres	165.2
2370	Male, age 61, both hemispheres	141.5
2058	Male, age 76, both hemispheres	137.1
2802	Male, age 74, right hemisphere	125.2
2378	Male, age 80, both hemispheres	115.3

Dorsal tegmental nucleus
nTPM: 149.2

2083	Male, age 80, both hemispheres	171.7
1936	Male, age 76, left hemisphere	155.3
2326	Male, age 74, both hemispheres	147.1
2358	Male, age 61, both hemispheres	122.9

Dorsolateral tegmental area
nTPM: 156.4

2360	Male, age 74, both hemispheres	185.1
2654	Male, age 80, both hemispheres	172.3
2751	Female, age 85, both hemispheres	172.0
2141	Male, age 76, both hemispheres	145.2
2337	Male, age 61, both hemispheres	107.6

Kolliker-Fuse nucleus
nTPM: 209.6

2315	Male, age 76, right hemisphere	267.4
2163	Male, age 80, both hemispheres	218.5
2793	Female, age 91, left hemisphere	142.9

Lateral lemniscal nuclei
nTPM: 171.5

2036	Male, age 80, both hemispheres	197.7
2169	Male, age 76, both hemispheres	190.5
2746	Female, age 85, left hemisphere	182.1
2453	Male, age 74, right hemisphere	145.9
2320	Female, age 91, left hemisphere	141.5

Lateral parabrachial nucleus
nTPM: 174.7

2139	Female, age 85, right hemisphere	210.9
2792	Male, age 76, both hemispheres	175.1
2013	Male, age 80, both hemispheres	157.5
2790	Male, age 61, both hemispheres	155.1

Lateral vestibular nucleus
nTPM: 169.2

2184	Male, age 76, both hemispheres	182.8
2311	Male, age 80, both hemispheres	155.6

Locus coeruleus
nTPM: 213.9

2123	Female, age 91, both hemispheres	249.8
2014	Male, age 55, unknown	245.1
2788	Male, age 74, unknown	214.7
2720	Male, age 74, both hemispheres	214.1
2317	Female, age 72, unknown	200.1
2162	Male, age 80, both hemispheres	192.6
1980	Male, age 76, both hemispheres	181.2

Medial parabrachial nucleus
nTPM: 161.1

2170	Female, age 91, both hemispheres	189.1
2854	Male, age 80, both hemispheres	169.9
2156	Male, age 61, both hemispheres	154.8
2786	Male, age 76, both hemispheres	147.3
2463	Female, age 85, right hemisphere	144.2

Medial periolivary nuclei
nTPM: 175.9

2791	Male, age 74, right hemisphere	218.8
2171	Female, age 85, both hemispheres	216.1
2063	Male, age 76, both hemispheres	203.8
2081	Male, age 80, both hemispheres	121.7
2458	Female, age 91, both hemispheres	119.3

Motor facial nucleus
nTPM: 168.1

2062	Male, age 76, both hemispheres	211.9
2365	Male, age 74, right hemisphere	198.5
2697	Female, age 85, both hemispheres	170.9
2327	Male, age 80, both hemispheres	149.7
2126	Male, age 61, both hemispheres	147.2
2795	Female, age 91, both hemispheres	130.4

Motor trigeminal nucleus
nTPM: 146.9

2309	Male, age 76, both hemispheres	158.9
2079	Male, age 80, both hemispheres	152.1
2560	Male, age 74, both hemispheres	148.1
2335	Male, age 61, both hemispheres	148.0
2321	Female, age 91, both hemispheres	127.3

Nuclei of the trapezoid body
nTPM: 185.8

2159	Male, age 61, both hemispheres	229.2
2735	Female, age 85, both hemispheres	211.2
1983	Male, age 76, both hemispheres	203.0
2166	Male, age 80, both hemispheres	158.8
2368	Female, age 91, both hemispheres	126.9

Nucleus raphe magnus
nTPM: 186.8

2160	Male, age 61, both hemispheres	239.2
2172	Female, age 85, both hemispheres	215.9
2455	Male, age 74, both hemispheres	201.0
2051	Male, age 76, both hemispheres	172.8
2328	Male, age 80, both hemispheres	156.0
2459	Female, age 91, both hemispheres	136.1

Paramedian reticular nucleus
nTPM: 200.0

2313	Male, age 61, both hemispheres	216.0
2050	Female, age 85, both hemispheres	215.4
2057	Male, age 80, both hemispheres	199.4
2052	Male, age 76, both hemispheres	169.2

Pedunculopontine tegmental nucleus
nTPM: 169.3

1921	Female, age 85, both hemispheres	183.8
2334	Male, age 80, both hemispheres	181.9
2338	Male, age 61, both hemispheres	142.2

Pontine nuclei
nTPM: 193.7

2787	Female, age 85, both hemispheres	231.1
1863	Male, age 55, unknown	226.9
2727	Male, age 61, right hemisphere	226.3
2109	Female, age 72, unknown	207.7
2454	Male, age 74, left hemisphere	199.6
1968	Male, age 76, left hemisphere	184.5
2319	Female, age 91, both hemispheres	144.5
2115	Male, age 80, left hemisphere	129.2

Pontine raphe nucleus
nTPM: 176.3

2122	Female, age 85, midline	198.3
2853	Male, age 76, midline	154.4

Posteroventral cochlear nucleus
nTPM: 148.8

2752	Female, age 85, both hemispheres	201.8
2070	Male, age 76, both hemispheres	173.6
2371	Male, age 61, both hemispheres	139.3
2135	Female, age 91, both hemispheres	119.2
2341	Male, age 80, both hemispheres	110.2

Principal sensory trigeminal nucleus
nTPM: 179.4

2158	Male, age 61, both hemispheres	215.6
2008	Male, age 80, left hemisphere	188.8
2324	Male, age 76, both hemispheres	177.8
2367	Female, age 91, left hemisphere	135.5

Reticular pontine nucleus, caudal
nTPM: 157.4

2092	Male, age 76, both hemispheres	190.0
2332	Male, age 80, right hemisphere	162.9
2559	Male, age 74, both hemispheres	155.5
2457	Female, age 91, both hemispheres	121.3

Reticular pontine nucleus, oral
nTPM: 167.5

2012	Female, age 85, both hemispheres	192.0
2361	Male, age 74, both hemispheres	191.5
1939	Male, age 80, both hemispheres	170.2
2167	Male, age 76, both hemispheres	148.2
2366	Female, age 91, both hemispheres	135.7

Reticulotegmental nucleus
nTPM: 155.8

2325	Male, age 76, both hemispheres	174.1
2322	Female, age 91, both hemispheres	153.3
2452	Male, age 74, both hemispheres	149.8
2333	Male, age 80, both hemispheres	146.1

Spinal trigeminal nucleus, oral
nTPM: 175.8

2737	Female, age 85, both hemispheres	194.5
1978	Male, age 76, both hemispheres	170.2
2164	Male, age 80, both hemispheres	162.8

Subcoeruleus area
nTPM: 166.4

2323	Female, age 91, left hemisphere	199.4
1958	Male, age 80, both hemispheres	194.2
2369	Female, age 85, left hemisphere	149.3
2089	Male, age 76, left hemisphere	147.1
2451	Male, age 74, both hemispheres	142.0

Superior olive
nTPM: 180.9

2364	Male, age 74, right hemisphere	240.0
1875	Female, age 85, both hemispheres	217.7
2020	Male, age 76, both hemispheres	169.6
2329	Male, age 80, right hemisphere	148.2
2561	Female, age 91, both hemispheres	128.8

Ventral periolivary nuclei
nTPM: 175.1

2165	Male, age 80, both hemispheres	189.6
2130	Female, age 85, both hemispheres	189.4
2023	Male, age 76, both hemispheres	181.7
2794	Female, age 91, left hemisphere	139.6

Ventrolateral tegmental area, A5 NE cell group
nTPM: 157.4

2840	Female, age 85, both hemispheres	183.9
2449	Male, age 74, both hemispheres	180.0
1959	Male, age 76, both hemispheres	173.0
2359	Male, age 61, both hemispheres	155.7
2161	Male, age 80, both hemispheres	143.9
2456	Female, age 91, both hemispheres	108.1

PONS - FANTOM5 CAGEⁱ

Max subtype Scaled Tags Per Millionⁱ Max subtype Scaled Tags Per Million is the value for the subtype with the largest average Scaled Tags Per Million across the subtype samples.	278.2

FANTOM5 sample id

Sample description

Scaled Tags Per Million

Locus coeruleus
Scaled Tags Per Million: 199.4

FF:10182-103D2	60 years, female	261.1
FF:10165-103B3	76 years, female	137.8

Pons
Scaled Tags Per Million: 278.2

FF:10033-101E6

18-54 years, mixed

278.2

PONS - Macaque RNA-seq

Max subtype nTPMⁱ Max subtype nTPM is the value for the subtype with the largest average nTPM across the subtype samples.	503.2

Macaque sample

Sample description

nTPM

Pons
nTPM: 503.2

Samples: 2

Max nTPM: 597.6
Min nTPM: 408.7

Sample A		597.6
Sample B		408.7

PONS - Pig RNA-seqⁱ

Max subtype nTPMⁱ Max subtype nTPM is the value for the subtype with the largest average nTPM across the subtype samples.	388.9

Pig sample

Sample description

nTPM

Pons
nTPM: 388.9

Samples: 3

Max nTPM: 442.8
Min nTPM: 320.9

Sample A	Female	402.9
Sample B	Female	320.9
Sample C	Male	442.8

PONS - Mouse RNA-seqⁱ

Max subtype nTPMⁱ Max subtype nTPM is the value for the subtype with the largest average nTPM across the subtype samples.	150.9

Mouse sample

Sample description

nTPM

Pons
nTPM: 150.9

Samples: 4

Max nTPM: 177.7
Min nTPM: 128.9

Sample A		177.7
Sample B		128.9
Sample C		160.5
Sample D		136.3

PONS - Rat RNA-seq

Max subtype nTPMⁱ Max subtype nTPM is the value for the subtype with the largest average nTPM across the subtype samples.	283.3

Rat sample

Sample description

nTPM

Pons
nTPM: 283.4

Samples: 4

Max nTPM: 308.7
Min nTPM: 265.0

Sample A		287.0
Sample A		308.7
Sample A		265.0
Sample A		272.7