Single cell type - EDN1 - The Human Protein Atlas

GENERAL INFORMATIONⁱ General description of the gene and the encoded protein(s) using information from HGNC and Ensembl, as well as predictions made by the Human Protein Atlas project.
Gene nameⁱ Official gene symbol, which is typically a short form of the gene name, according to HGNC.	EDN1
Gene descriptionⁱ Full gene name according to HGNC.	Endothelin 1
Protein classⁱ Assigned HPA protein class(es) for the encoded protein(s). Read more	Cancer-related genes Candidate cardiovascular disease genes Disease related genes Human disease related genes Plasma proteins
Predicted locationⁱ All transcripts of all genes have been analyzed regarding the location(s) of corresponding protein based on prediction methods for signal peptides and transmembrane regions. Genes with at least one transcript predicted to encode a secreted protein, according to prediction methods or to UniProt location data, have been further annotated and classified with the aim to determine if the corresponding protein(s) are secreted or actually retained in intracellular locations or membrane-attached. Remaining genes, with no transcript predicted to encode a secreted protein, will be assigned the prediction-based location(s). The annotated location overrules the predicted location, so that a gene encoding a predicted secreted protein that has been annotated as intracellular will have intracellular as the final location.	Secreted
Number of transcriptsⁱ Number of protein-coding transcripts from the gene as defined by Ensembl.	1

HUMAN PROTEIN ATLAS INFORMATIONⁱ Summary of RNA expression and protein localization based on data generated within the Human Protein Atlas project.
Single cell type expression clusterⁱ The RNA data was used to cluster genes according to their expression across single cell types. Clusters contain genes that have similar expression patterns, and each cluster has been manually annotated to describe common features in terms of function and specificity.	Endothelial cells - Angiogenesis & vascular immunity (mainly)
Single cell type specificityⁱ The RNA specificity category is based on mRNA expression levels in the analyzed cell types based on scRNA-seq data from normal tissues. The categories include: cell type enriched, group enriched, cell type enhanced, low cell type specificity and not detected.	Cell type enhanced (Epididymal basal cells, Enteric stem cells, Endometrial glandular cells, Pancreatic duct cells, Breast secretory cells, Endometrial luminal cells, Alveolar cells type 1, Endometrial secretory cells, Epididymal efferent duct absorptive cells, Vascular endothelial cells, Endometrial ciliated cells, Basal keratinocytes, Enteric transient amplifying cells, Colonocytes, Enterocytes)
Tau specificity scoreⁱ Tau specificity score is a numerical indicator of the specificity of the gene expression across cells or tissues. The value ranges from 0 and 1, where 0 indicates identical expression across all cells/tissue types, while 1 indicates expression in a single cell/tissue type.	0.68
Brain cluster specificityⁱ The RNA specificity category is based on mRNA expression levels in the analyzed cell cluster types based on snRNA-seq data from brain samples. The categories include: cell type enriched, group enriched, cell type enhanced, low cell type specificity and not detected. "cell type" refer to one of the 34 cluster names used to summarize the 2.5 million cells included in the brain cell representation.	Cell type enriched (Endothelial cell)
Immune cell specificityⁱ The RNA specificity category is based on mRNA expression levels in the analyzed samples based on data from HPA. The categories include: cell type enriched, group enriched, cell type enhanced, low cell type specificity and not detected.	Immune cell enriched (non-classical monocyte)
Protein evidenceⁱ Evidence score for genes based on UniProt protein existence (UniProt evidence); neXtProt protein existence (neXtProt evidence);and a Human Protein Atlas antibody- or RNA based score (HPA evidence). The avaliable scores are evidence at protein level, evidence at transcript level, no evidence, or not avaliable.	Evidence at protein level
Extracellular locationⁱ A possible secretion of the protein was predicted based on N-terminal signal sequence (signal peptide) predictions and transmembrane region predictions, and subsequently annotated for its local or systemic secretion based on literature, function, and protein and RNA expression data.	Protein predicted to be secreted

ASSAYSⁱ

Single cell type

Cell type enrichment

Single nuclei - Brain

Immune cells

Expression clustering

SINGLE CELL TYPESⁱ A summary of normalized single cell RNA (nCPM) from all single cell types. Color-coding is based on cell type groups, each consisting of cell types with functional features in common. * Single nuclei Read more
Single cell type groups RNA single cell type group specificity: Cell type enhanced (Basal cells, Stem cells, Glandular cells, Ductal cells, Secretory cells)

TISSUE CELL TYPEⁱ The tissue cell type section provides a cell type specificity prediction for all protein coding genes. More information on the data available can be found here and a description of the analysis here.
	Core cell typesⁱ The core cell type table displays data for a panel of eight cell types that are found in many tissues. Colored dots indicate that the selected gene has core cell type specificity in the indicated tissue. Grey dots indicate that cell type is present within that tissue, but the selected gene is not predicted to be enriched there. Category Alphabetical Enrichment Tissue specific cell typesⁱ This plot shows the enrichment score of the selected gene in all non-core cell types found within the profiled tissues, allowing comparisons between different cell types within different tissues. An enlarged symbol indicates classification of the gene as cell type enriched in the corresponding cell type.

SINGLE NUCLEI BRAINⁱ

Single nuclei cluster types

RNA brain cluster specificity: Cell type enriched (Endothelial cell)

IMMUNE CELL TYPE EXPRESSION (RNA)ⁱ Blood cell type expression overview shows RNA-seq data from three different sources: Internally generated Human Protein Atlas HPA data, as well as data generated by Monaco et al and Schmiedel et al). Color-coding is based on blood cell type lineages, including B-cells, T-cells, NK-cells, monocytes, granulocytes and dendritic cells as well as total PBMC. To access sample data, click on tissue name or bar.
HPA datasetⁱ The resulting transcript expression values calculated as nTPM, resulting from the internal normalization pipeline for 18 immune cell types and total peripheral blood mononuclear cells (PBMC). Lineage Expression Alphabetical Immune cell type specificity: Immune cell enriched (non-classical monocyte)
Monaco datasetⁱ The transcript expression values (nTPM) resulting from the internal normalization pipeline are visualized for 29 blood cell types and total peripheral blood mononuclear cells (PBMC) from Monaco et al. Lineage Expression Alphabetical
Schmiedel datasetⁱ Transcript Per Million (TPM) values are visualized for 15 blood cell types from the Schmiedel et al. dataset. Lineage Expression Alphabetical

EXPRESSION CLUSTERING & CORRELATIONⁱ

SINGLE CELL TYPE

EDN1 is part of cluster 32 Endothelial cells - Angiogenesis & vascular immunity with confidenceⁱ 1
373 genes in cluster

Go to interactive expression cluster page

15 nearest neighbours based on single cell RNA expression

Neighbourⁱ Gene name according to HGNC.	Descriptionⁱ Gene description according to HGNC.	Correlationⁱ Correlation between the selected gene and neighboring gene. Correlation is calculated as Spearman correlation in PCA space based on the RNA-seq expression data.	Clusterⁱ ID of the expression cluster of the neighboring gene.
CLEC14A	C-type lectin domain containing 14A	0.6878	32
DHH	Desert hedgehog signaling molecule	0.6854	32
SEMA3G	Semaphorin 3G	0.6839	32
HEY1	Hes related family bHLH transcription factor with YRPW motif 1	0.6822	32
WNT2B	Wnt family member 2B	0.6797	32
DEPP1	DEPP1 autophagy regulator	0.6661	32
JAG2	Jagged canonical Notch ligand 2	0.6459	32
CTTNBP2NL	CTTNBP2 N-terminal like	0.6404	32
PKN3	Protein kinase N3	0.6377	32
SLC9A3R2	SLC9A3 regulator 2	0.6337	32
MMRN2	Multimerin 2	0.6300	32
FAM107A	Family with sequence similarity 107 member A	0.6211	32
S1PR1	Sphingosine-1-phosphate receptor 1	0.6062	32
SRP14	Signal recognition particle 14	0.6049	32
RAMP2	Receptor activity modifying protein 2	0.6043	32

IMMUNE CELLS

EDN1 is part of cluster 25 Non-specific - Unknown function with confidenceⁱ 0.86
355 genes in cluster

Go to interactive expression cluster page

15 nearest neighbours based on single cell RNA expression

Neighbourⁱ Gene name according to HGNC.	Descriptionⁱ Gene description according to HGNC.	Correlationⁱ Correlation between the selected gene and neighboring gene. Correlation is calculated as Spearman correlation in PCA space based on the RNA-seq expression data.	Clusterⁱ ID of the expression cluster of the neighboring gene.
SPG11	SPG11 vesicle trafficking associated, spatacsin	1.0000	25
ACAP3	ArfGAP with coiled-coil, ankyrin repeat and PH domains 3	1.0000	25
NECAB3	N-terminal EF-hand calcium binding protein 3	0.9762	25
FNDC10	Fibronectin type III domain containing 10	0.9762	25
RAB11B	RAB11B, member RAS oncogene family	0.9762	25
BEGAIN	Brain enriched guanylate kinase associated	0.9762	29
RMDN1	Regulator of microtubule dynamics 1	0.9762	25
CRELD1	Cysteine rich with EGF like domains 1	0.9762	25
GXYLT1	Glucoside xylosyltransferase 1	0.9762	25
PPCDC	Phosphopantothenoylcysteine decarboxylase	0.9762	25
IL18BP	Interleukin 18 binding protein	0.9762	25
POLDIP2	DNA polymerase delta interacting protein 2	0.9762	25
INSIG2	Insulin induced gene 2	0.9762	25
PEDS1-UBE2V1	PEDS1-UBE2V1 readthrough	0.9762	25
ORMDL2	ORMDL sphingolipid biosynthesis regulator 2	0.9762	25