Brain tissue expression of RUNDC3A in pons

PONS - Expression summary

Mouse brain, Intensity
Ponsⁱ

158.501

RNA expressionⁱ

HPA Brain:	141.0	nTPM
FANTOM5:	96.7	Scaled Tags Per Million

Macaque:	72.1	nTPM
Mouse:	115.3	nTPM
Rat:	119.0	nTPM
Pig:	171.4	nTPM

PONS - SINGLE NUCLEI RNA EXPRESSIONⁱ RNA expression in the single nuclei cluster types identified in this brain region visualized by a UMAP plot (top) and a bar chart (to the right). - UMAP PLOT visualizes the cells in each cluster; where each dot corresponds to a cell. For each individual cell, mouseover reveals read count and which cluster the cell belongs to. Hovering over a cluster name will highlight the corresponding cluster in the bar chart two the right. There are 2 options for color schemes: 1) cell type color, which is based on cell type groups used in the Single cell type part of the Single cell resource and 2) cluster color, which assigns a unique color to each cluster. There are 3 options for cell intensities: 1) Intensity, which color the individual cells according to % of max (log2(read_count+1)/log2(max(read_count)+1)100) in five different bins (<1%,<25%,<50%,<75%,≥75%) 2) Interval, which color the individual cells according to fixed interval of read count (0,1,2-4,5-9,>10) 3) Hexagon, which color the hexagon on average read count of all cells inside the hexagon - The BAR CHART* shows RNA expression (nTPM) in each cluster type. Hovering over the cluster name reveals nTPM value and number of included cells. Hovering over a bar highlights the corresponding cluster in the UMAP plot above. Color-coding can be toggled on the top of the page, between 2 options: 1) cell type color, which is based on cell type groups used in the Single cell type part of the Single cell resource and 2) cluster color, which assigns a unique color to each cluster.

SINGLE NUCLEI BRAIN - PONS, NEURONSⁱ

Cell type color

Cluster color

Colored according to cell type group

Read count filter: 0

Cluster		Cell type
	c-1	Excitatory (VGLUT1/2)
	c-2	Excitatory (VGLUT1) ETV1+ Z...
	c-3	Excitatory (VGLUT1/2)
	c-4	Excitatory (VGLUT1) ETV1+ Z...
	c-5	Excitatory (VGLUT1/2)
	c-6	Excitatory (VGLUT1/2)
	c-7	Excitatory (VGLUT1/2)
	c-8	Inhibitory (GAD1/2) SLC6A5+
	c-9	Excitatory (VGLUT2)
	c-10	Excitatory (VGLUT1/2)
	c-11	Excitatory (VGLUT1/2)
	c-12	Unassigned
	c-13	Inhibitory (GAD1/2) RORB+ K
	c-14	Inhibitory (GAD1/2) SST+
	c-15	Excitatory (VGLUT1) ETV1+ Z...
	c-16	Excitatory (VGLUT2)
	c-17	Excitatory (VGLUT1/2)
	c-18	Inhibitory (GAD1/2)
	c-19	Inhibitory (GAD1) PENK+ SST
	c-20	Excitatory (VGLUT2) FOXP2+
	c-21	Excitatory (VGLUT2) LMX1A+
	c-22	Excitatory (VGLUT2) MEIS2+
	c-23	Inhibitory (GAD1/2) SLC6A5+...
	c-24	Inhibitory (GAD1/2) SLC6A5+...
	c-25	Serotonergic SLC6A4+ SLC18A...
	c-26	Inhibitory (GAD1/2) PVALB+ ...
	c-27	Excitatory (VGLUT2)
	c-28	(VGLUT3) PENK+
	c-29	MEIS2+
	c-30	LMX1A+
	c-31	Inhibitory (GAD1/2) TAC1+
	c-32	Excitatory (VGLUT2) PVALB+
	c-33	Inhibitory (GAD1/2) PVALB+ ...
	c-34	Inhibitory (GAD1/2)
	c-35	Excitatory (VGLUT2) SST+ ME...
	c-36	LC Noradrenergic SLC6A2+ SL...
	c-37	Excitatory (VGLUT2) CRH+ FO...
	c-38	Inhibitory (GAD1/2) PVALB+ ...
	c-39	Excitatory (VGLUT2)
	c-40	Excitatory (VGLUT2) PVALB+
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This data was generated by Siletti K et al. (2023), re-processed by HPA (see methods) and simplified for global overview

SINGLE NUCLEI BRAIN - PONS, NON-NEURONALⁱ

Cell type color

Cluster color

Colored according to cell type group

Read count filter: 0

Cluster		Cell type
	c-1	Oligodendrocytes
	c-2	Oligodendrocytes
	c-3	Oligodendrocyte progenitor ...
	c-4	Oligodendrocytes
	c-5	Oligodendrocytes
	c-6	Oligodendrocytes
	c-7	Microglia
	c-8	Astrocytes
	c-9	Oligodendrocytes
	c-10	Astrocytes (CD44+)
	c-11	Oligodendrocytes
	c-12	Fibroblasts
	c-13	Vascular endothelial cells
	c-14	Pericytes
	c-15	Ependymal cells
	c-16	Lymphocytes
	c-17	Macrophages
	c-18	Microglia (SPP1+ HIF1A+)
	c-19	Bergmann glia
	c-20	Immature oligodendrocytes
	c-21	Oligodendrocyte progenitor ...
	c-22	Vascular smooth muscle cell
	c-23	Monocytes
	c-24	Immature oligodendrocytes
	c-25	Fibroblasts (BNC2+ KCNMA1+ ...
	c-26	Choroid plexus epithelial c...
	c-27	Macrophage Microglia double
	c-28	OPC & lymphocytes doublets
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This data was generated by Siletti K et al. (2023), re-processed by HPA (see methods) and simplified for global overview

PONS - Mouse brain profileⁱ Protein expression in mouse brain using immunofluorescence is reported as fluorescence intensity. , antibody HPA023548
Pons mean intensity	158.501
	intensityⁱ Protein expression in mouse brain using immunofluorescence is reported as mean fluorescence intensity.	FRONT BACK
Barrington's nucleus	-
Koelliker-fuse nucleus	-
Motor tregiminal nucleus	35.238
Parabrachial nucleus	17.508
Principle sensory trigeminal nucleus	11.898
Locus coeruleus	158.501
Pontine nucleus	13.253
Vestibular nucleus	23.713
Pontine reticular nucleus, oral	14.530
Lateral lemniscus	8.479
Superior paraolivary nucleus	9.478

PONS - HPA Brain Stereo-seq

PONS - HPA Brain RNA-seq

Max subtype nTPMⁱ Max subtype nTPM is the value for the subtype with the largest average nTPM across the subtype samples.	141.0

Sample id

Sample description

nTPM

Anterior cochlear nucleus, ventral
nTPM: 71.4

1984	Male, age 76, both hemispheres	91.4
2301	Female, age 85, both hemispheres	77.5
2090	Male, age 80, both hemispheres	61.5
2188	Female, age 91, both hemispheres	55.2

Dorsal cochlear nucleus
nTPM: 82.3

2058	Male, age 76, both hemispheres	98.5
2802	Male, age 74, right hemisphere	89.4
2370	Male, age 61, both hemispheres	87.7
2187	Female, age 91, both hemispheres	76.2
2378	Male, age 80, both hemispheres	59.6

Dorsal tegmental nucleus
nTPM: 79.7

2083	Male, age 80, both hemispheres	91.7
1936	Male, age 76, left hemisphere	83.7
2358	Male, age 61, both hemispheres	76.6
2326	Male, age 74, both hemispheres	66.9

Dorsolateral tegmental area
nTPM: 68.7

2141	Male, age 76, both hemispheres	90.5
2654	Male, age 80, both hemispheres	85.5
2751	Female, age 85, both hemispheres	76.4
2360	Male, age 74, both hemispheres	54.8
2337	Male, age 61, both hemispheres	36.2

Kolliker-Fuse nucleus
nTPM: 81.5

2163	Male, age 80, both hemispheres	121.8
2793	Female, age 91, left hemisphere	71.2
2315	Male, age 76, right hemisphere	51.4

Lateral lemniscal nuclei
nTPM: 114.1

2453	Male, age 74, right hemisphere	143.2
2169	Male, age 76, both hemispheres	140.0
2746	Female, age 85, left hemisphere	104.7
2036	Male, age 80, both hemispheres	92.0
2320	Female, age 91, left hemisphere	90.7

Lateral parabrachial nucleus
nTPM: 80.3

2139	Female, age 85, right hemisphere	118.4
2790	Male, age 61, both hemispheres	98.8
2013	Male, age 80, both hemispheres	72.3
2792	Male, age 76, both hemispheres	31.7

Lateral vestibular nucleus
nTPM: 94.3

2184	Male, age 76, both hemispheres	115.6
2311	Male, age 80, both hemispheres	73.0

Locus coeruleus
nTPM: 141.0

2123	Female, age 91, both hemispheres	178.5
2720	Male, age 74, both hemispheres	164.8
2014	Male, age 55, unknown	138.6
2162	Male, age 80, both hemispheres	137.6
1980	Male, age 76, both hemispheres	129.2
2317	Female, age 72, unknown	127.0
2788	Male, age 74, unknown	111.4

Medial parabrachial nucleus
nTPM: 90.4

2156	Male, age 61, both hemispheres	118.8
2786	Male, age 76, both hemispheres	86.5
2463	Female, age 85, right hemisphere	82.8
2170	Female, age 91, both hemispheres	82.3
2854	Male, age 80, both hemispheres	81.7

Medial periolivary nuclei
nTPM: 113.4

2171	Female, age 85, both hemispheres	171.4
2063	Male, age 76, both hemispheres	156.0
2791	Male, age 74, right hemisphere	87.1
2458	Female, age 91, both hemispheres	82.0
2081	Male, age 80, both hemispheres	70.6

Motor facial nucleus
nTPM: 115.7

2062	Male, age 76, both hemispheres	174.0
2327	Male, age 80, both hemispheres	121.6
2365	Male, age 74, right hemisphere	113.0
2795	Female, age 91, both hemispheres	97.6
2697	Female, age 85, both hemispheres	97.4
2126	Male, age 61, both hemispheres	90.9

Motor trigeminal nucleus
nTPM: 93.8

2079	Male, age 80, both hemispheres	104.6
2560	Male, age 74, both hemispheres	101.9
2335	Male, age 61, both hemispheres	99.2
2309	Male, age 76, both hemispheres	86.1
2321	Female, age 91, both hemispheres	77.0

Nuclei of the trapezoid body
nTPM: 136.5

2735	Female, age 85, both hemispheres	168.5
1983	Male, age 76, both hemispheres	160.3
2159	Male, age 61, both hemispheres	154.2
2166	Male, age 80, both hemispheres	108.3
2368	Female, age 91, both hemispheres	91.1

Nucleus raphe magnus
nTPM: 136.1

2172	Female, age 85, both hemispheres	185.6
2160	Male, age 61, both hemispheres	179.0
2455	Male, age 74, both hemispheres	138.9
2051	Male, age 76, both hemispheres	117.5
2328	Male, age 80, both hemispheres	113.1
2459	Female, age 91, both hemispheres	82.5

Paramedian reticular nucleus
nTPM: 105.5

2313	Male, age 61, both hemispheres	127.8
2050	Female, age 85, both hemispheres	127.0
2057	Male, age 80, both hemispheres	110.3
2052	Male, age 76, both hemispheres	57.0

Pedunculopontine tegmental nucleus
nTPM: 85.6

2334	Male, age 80, both hemispheres	97.4
1921	Female, age 85, both hemispheres	97.3
2338	Male, age 61, both hemispheres	62.1

Pontine nuclei
nTPM: 122.2

2787	Female, age 85, both hemispheres	181.2
1968	Male, age 76, left hemisphere	151.7
2727	Male, age 61, right hemisphere	148.2
1863	Male, age 55, unknown	140.1
2109	Female, age 72, unknown	115.2
2454	Male, age 74, left hemisphere	91.9
2115	Male, age 80, left hemisphere	85.8
2319	Female, age 91, both hemispheres	63.5

Pontine raphe nucleus
nTPM: 110.3

2122	Female, age 85, midline	127.8
2853	Male, age 76, midline	92.8

Posteroventral cochlear nucleus
nTPM: 68.3

2752	Female, age 85, both hemispheres	120.3
2070	Male, age 76, both hemispheres	84.8
2371	Male, age 61, both hemispheres	74.9
2341	Male, age 80, both hemispheres	36.3
2135	Female, age 91, both hemispheres	25.2

Principal sensory trigeminal nucleus
nTPM: 115.2

2158	Male, age 61, both hemispheres	171.8
2008	Male, age 80, left hemisphere	115.4
2324	Male, age 76, both hemispheres	105.2
2367	Female, age 91, left hemisphere	68.3

Reticular pontine nucleus, caudal
nTPM: 97.8

2332	Male, age 80, right hemisphere	121.8
2092	Male, age 76, both hemispheres	105.1
2457	Female, age 91, both hemispheres	88.9
2559	Male, age 74, both hemispheres	75.5

Reticular pontine nucleus, oral
nTPM: 88.4

2012	Female, age 85, both hemispheres	107.5
2361	Male, age 74, both hemispheres	101.1
2167	Male, age 76, both hemispheres	89.1
1939	Male, age 80, both hemispheres	81.9
2366	Female, age 91, both hemispheres	62.5

Reticulotegmental nucleus
nTPM: 102.6

2452	Male, age 74, both hemispheres	118.6
2325	Male, age 76, both hemispheres	112.9
2333	Male, age 80, both hemispheres	90.5
2322	Female, age 91, both hemispheres	88.3

Spinal trigeminal nucleus, oral
nTPM: 109.4

2164	Male, age 80, both hemispheres	110.1
2737	Female, age 85, both hemispheres	109.4
1978	Male, age 76, both hemispheres	108.9

Subcoeruleus area
nTPM: 99.2

2323	Female, age 91, left hemisphere	126.8
2089	Male, age 76, left hemisphere	112.7
1958	Male, age 80, both hemispheres	100.1
2451	Male, age 74, both hemispheres	95.7
2369	Female, age 85, left hemisphere	60.5

Superior olive
nTPM: 102.5

1875	Female, age 85, both hemispheres	116.0
2329	Male, age 80, right hemisphere	107.0
2020	Male, age 76, both hemispheres	97.7
2364	Male, age 74, right hemisphere	96.0
2561	Female, age 91, both hemispheres	95.8

Ventral periolivary nuclei
nTPM: 118.7

2130	Female, age 85, both hemispheres	166.8
2165	Male, age 80, both hemispheres	137.5
2794	Female, age 91, left hemisphere	92.4
2023	Male, age 76, both hemispheres	78.1

Ventrolateral tegmental area, A5 NE cell group
nTPM: 100.2

2449	Male, age 74, both hemispheres	130.4
1959	Male, age 76, both hemispheres	106.7
2359	Male, age 61, both hemispheres	97.1
2840	Female, age 85, both hemispheres	94.8
2161	Male, age 80, both hemispheres	92.3
2456	Female, age 91, both hemispheres	80.2

PONS - FANTOM5 CAGEⁱ

Max subtype Scaled Tags Per Millionⁱ Max subtype Scaled Tags Per Million is the value for the subtype with the largest average Scaled Tags Per Million across the subtype samples.	96.7

FANTOM5 sample id

Sample description

Scaled Tags Per Million

Locus coeruleus
Scaled Tags Per Million: 50.5

FF:10165-103B3	76 years, female	53.2
FF:10182-103D2	60 years, female	47.8

Pons
Scaled Tags Per Million: 96.7

FF:10033-101E6

18-54 years, mixed

96.7

PONS - Macaque RNA-seq

Max subtype nTPMⁱ Max subtype nTPM is the value for the subtype with the largest average nTPM across the subtype samples.	72.1

Macaque sample

Sample description

nTPM

Pons
nTPM: 72.1

Samples: 2

Max nTPM: 89.1
Min nTPM: 55.1

Sample A		89.1
Sample B		55.1

PONS - Pig RNA-seqⁱ

Max subtype nTPMⁱ Max subtype nTPM is the value for the subtype with the largest average nTPM across the subtype samples.	171.4

Pig sample

Sample description

nTPM

Pons
nTPM: 171.4

Samples: 3

Max nTPM: 235.7
Min nTPM: 125.9

Sample A	Female	235.7
Sample B	Female	125.9
Sample C	Male	152.7

PONS - Mouse RNA-seqⁱ

Max subtype nTPMⁱ Max subtype nTPM is the value for the subtype with the largest average nTPM across the subtype samples.	115.3

Mouse sample

Sample description

nTPM

Pons
nTPM: 115.3

Samples: 4

Max nTPM: 125.2
Min nTPM: 107.0

Sample A		107.0
Sample B		125.2
Sample C		111.7
Sample D		117.3

PONS - Rat RNA-seq

Max subtype nTPMⁱ Max subtype nTPM is the value for the subtype with the largest average nTPM across the subtype samples.	119.0

Rat sample

Sample description

nTPM

Pons
nTPM: 118.9

Samples: 4

Max nTPM: 123.7
Min nTPM: 115.0

Sample A		123.7
Sample A		116.0
Sample A		115.0
Sample A		121.1