Fields »

SUBCELLULAR LOCATION

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Main location(s)

Nuclear membrane

Additional location(s)

Nucleoli, Cytoplasm 

Staining summary

Mainly localized to the nuclear membrane. In addition localized to nucleoli and cytoplasm.

Reliability (APE)

Low

Antibodies in assay

HPA040755, HPA040868
 

Antibody HPA040755

 

Antibody HPA040868

 

         Cell line A-431

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Location(s)

Nucleoli, Nuclear membrane  

Staining characteristics

  

Staining intensity

Weak Negative 

Validation

One/multiple location(s) with no available experimental gene/protein characterization data.
 
No staining.
 

Comment

  

         Cell line MCF-7

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Location(s)

Nucleoli, Nuclear membrane   

Staining characteristics

   

Staining intensity

Moderate   

Validation

One/multiple location(s) with no available experimental gene/protein characterization data.
   

Comment

   

         Cell line U-2 OS

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Location(s)

Nuclear membrane, Cytoplasm  

Staining characteristics

Aggregate  

Staining intensity

Strong Negative 

Validation

One/multiple location(s) with no available experimental gene/protein characterization data.
 
No staining.
 

Comment

  

         Cell line U-251 MG

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Location(s)

   

Staining characteristics

   

Staining intensity

  Negative 

Validation

  
No staining.
 

Comment

   

         Antibody summary


Immunofluorescent staining of human cell line MCF-7 shows positivity in nucleoli & nuclear membrane.
 

Immunofluorescent staining of human cell line A-431 shows no positivity.
 

Summary

Staining of nuclear membrane in all three cell lines with additional staining of nucleoli in MCF-7 and A-431 and cytoplasm in U-2 OS.   No staining was observed in any of the three cell lines.  

Validation

The subcellular location is partly supported by literature or no literature is available.
 
The subcellular location is partly supported by literature or no literature is available.
 

Dictionary

Cytoplasm
Nuclear membrane
Nucleoli