Fields »

SUBCELLULAR LOCATION

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Main location(s)

Endoplasmic reticulum

Additional location(s)

Cytoskeleton (Intermediate filaments), Aggresome 

Staining summary

Staining of the endoplasmic reticulum in all three cell lines, with additional staining of cytoskeleton(intermediate filaments) in U-251 MG and U-2 OS.

Reliability (Single)

Antibodies in assay

HPA014059
 

Antibody HPA014059

 

         Cell line A-431

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Location(s)

Endoplasmic reticulum 

Staining characteristics

 

Staining intensity

Moderate 

Validation

One/multiple location(s) supported by experimental gene/protein characterization data.
 

Comment

 

         Cell line U-2 OS

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Location(s)

Endoplasmic reticulum, Cytoskeleton (Intermediate filaments), Aggresome 

Staining characteristics

 

Staining intensity

Moderate 

Validation

Multiple locations partly supported (at least one) by experimental gene/protein characterization data.
 

Comment

 

         Cell line U-251 MG

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Location(s)

Endoplasmic reticulum, Cytoskeleton (Intermediate filaments) 

Staining characteristics

 

Staining intensity

Moderate 

Validation

Multiple locations partly supported (at least one) by experimental gene/protein characterization data.
 

Comment

 

         Antibody summary


Immunofluorescent staining of human cell line A-431 shows positivity in endoplasmic reticulum.
 

Summary

Staining of the endoplasmic reticulum in all three cell lines, with additional staining of cytoskeleton(intermediate filaments) in U-251 MG and U-2 OS.  

Validation

The subcellular location is supported by literature.
 

Dictionary

Cell overview
Intermediate filaments
Endoplasmic reticulum