Fields »

SUBCELLULAR LOCATION

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Main location(s)

Nucleus, Cytoplasm

Additional location(s)

 

Staining summary

Localized to nucleus and cytoplasm.

Reliability (APE)

Medium

Antibodies in assay

CAB012423, HPA037363
 

Antibody CAB012423

 

Antibody HPA037363

 

         Cell line A-431

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Location(s)

Nucleus, Cytoplasm, Cytoskeleton (Intermediate filaments) Nucleus but not nucleoli, Cytoplasm 

Staining characteristics

  

Staining intensity

Moderate Weak 

Validation

Multiple locations partly supported (at least one) by experimental gene/protein characterization data.
 
One/multiple location(s) supported by experimental gene/protein characterization data.
 

Comment

  

         Cell line U-2 OS

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Location(s)

Nucleoli, Cytoplasm   

Staining characteristics

   

Staining intensity

Moderate   

Validation

Multiple locations partly supported (at least one) by experimental gene/protein characterization data.
   

Comment

   

         Cell line U-251 MG

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Location(s)

Cytoplasm   

Staining characteristics

   

Staining intensity

Weak   

Validation

Multiple locations partly supported (at least one) by experimental gene/protein characterization data.
   

Comment

   

         Antibody summary


Immunofluorescent staining of human cell line U-2 OS shows positivity in nucleoli & cytoplasm.
 

Immunofluorescent staining of human cell line A-431 shows positivity in nucleus but not nucleoli & cytoplasm.
 

Summary

Staining of cytoplasm in all three cell lines with additional staining of nucleus in A-431 and U-2 OS and of cytoskeleton (intermediate filaments) in A-431.   Staining of nucleus and cytoplasm in A-431.  

Validation

The subcellular location is supported by literature.
 
The subcellular location is supported by literature.
 

Dictionary

Cytoplasm
Nucleus