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SUBCELLULAR LOCATION

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Main location(s)

Plasma membrane

Additional location(s)

Cytoplasm, Cytoskeleton (Actin filaments) 

Staining summary

Staining of plasma membrane and cytoplasm in all three cell lines with additional staining of cytoskeleton (actin filaments) in A-431 and U-2 OS.

Reliability (Single)

Antibodies in assay

HPA023871
 

Antibody HPA023871

 

         Cell line A-431

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Location(s)

Plasma membrane, Cytoplasm, Cytoskeleton (Actin filaments) 

Staining characteristics

 

Staining intensity

Strong 

Validation

Multiple locations partly supported (at least one) by experimental gene/protein characterization data.
 

Comment

 

         Cell line U-2 OS

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Location(s)

Plasma membrane, Cytoplasm, Cytoskeleton (Actin filaments) 

Staining characteristics

 

Staining intensity

Strong 

Validation

Multiple locations partly supported (at least one) by experimental gene/protein characterization data.
 

Comment

 

         Cell line U-251 MG

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Location(s)

Plasma membrane, Cytoplasm 

Staining characteristics

 

Staining intensity

Strong 

Validation

Multiple locations partly supported (at least one) by experimental gene/protein characterization data.
 

Comment

 

         Antibody summary


Immunofluorescent staining of human cell line U-2 OS shows positivity in plasma membrane, cytoplasm & cytoskeleton (actin filaments).
 

Summary

Staining of plasma membrane and cytoplasm in all three cell lines with additional staining of cytoskeleton (actin filaments) in A-431 and U-2 OS.  

Validation

The subcellular location is supported by literature.
 

Dictionary

Cytoplasm
Actin filaments
Plasma membrane