ATP11A

ANTIBODY AND ANTIGEN INFORMATION

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Antibody HPA035583

 

Antibody HPA035584

 

ANTIBODY INFORMATION

Provider

Atlas Antibodies
Sigma-Aldrich
 Atlas Antibodies
Sigma-Aldrich
 

Product name

HPA035583 HPA035584 

Host species

Rabbit Rabbit 

Clonality

pAb pAb 

Purity

Affinity purified using the PrEST-antigen as affinity ligand Affinity purified using the PrEST-antigen as affinity ligand 

Released in version

7 7 

ANTIGEN INFORMATION

Antigen

Recombinant protein fragment Recombinant protein fragment 

Length (aa)

92 95 

Antigen sequence

SATGEIYLFCKGADSSIFPRVIEGKVDQIRARVERNAVEGLRTLCVAYKR
LIQEEYEGICKLLQAAKVALQDREKKLAEAYEQIEKDLTLLG
 
TCYACKLFRRNTQLLELTTKRIEEQSLHDVLFELSKTVLRHSGSLTRDNL
SGLSADMQDYGLIIDGAALSLIMKPREDGSSGNYRELFLEICRSC
 

Matching transcripts

ATP11A-001 - ENSP00000420387 [100%]
ATP11A-003 - ENSP00000396374 [100%]
ATP11A-201 - ENSP00000283558 [100%]
ATP11A-202 - ENSP00000364781 [100%]
ATP11A-203 - ENSP00000364796 [100%]
ATP11A-011 - ENSP00000420696 [84%]
 ATP11A-001 - ENSP00000420387 [100%]
ATP11A-003 - ENSP00000396374 [100%]
ATP11A-011 - ENSP00000420696 [100%]
ATP11A-201 - ENSP00000283558 [100%]
ATP11A-202 - ENSP00000364781 [100%]
ATP11A-203 - ENSP00000364796 [100%]
 

Other gene match

  

ANTIBODY VALIDATION

         Immunohistochemistry

Image

  

Description

Immunohistochemical staining of human kidney shows strong membranous and cytoplasmic positivity in cells in tubules.
More information
 Immunohistochemical staining of human kidney shows strong cytoplasmic and membranous positivity in distal tubules.
More information
 

Retrieval method

HIER pH6 HIER pH6 

Antibody dilution

1:200 1:90 

Validation IH

Uncertain: Two (or more) antibodies yielding dissimilar or partly similar staining patterns and there is no available gene/protein characterization data or only limited gene/protein characterization data Uncertain: Two (or more) antibodies yielding dissimilar or partly similar staining patterns and there is no available gene/protein characterization data or only limited gene/protein characterization data 

         Western Blot

Image

  

Description

Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT-4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil
More information
  

Target mass (kDa)

135.8, 129.8, 106.5, 39.5 135.8, 129.8, 106.5, 39.5 

Antibody dilution

1:250 1:250 

Validation WB

Uncertain: No bands detected Non-supportive: Weak band of predicted size but with additional bands of higher intensity also present 

         Protein array

Image

  

Description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
More information
 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
More information
 

Antibody dilution

1:3000 1:3000 

Validation PA

Uncertain: Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%). Supportive: Pass with single peak corresponding to interaction only with its own antigen. 
 

ANTIGEN VIEW

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ATP11A-001
 
ATP11A-003
 
ATP11A-011
 
ATP11A-201
 
ATP11A-202
 
ATP11A-203