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SUBCELLULAR LOCATION

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Main location(s)

Nucleus but not nucleoli, Cytoplasm

Additional location(s)

Golgi apparatus 

Staining summary

Staining of cytoplasm and nucleus in A-431 and U-251 MG with additional staining of Golgi in A-431.

Reliability (Single)

Antibodies in assay

HPA014757
 

Antibody HPA014757

 

         Cell line A-431

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Location(s)

Nucleus but not nucleoli, Cytoplasm, Golgi apparatus 

Staining characteristics

 

Staining intensity

Moderate 

Validation

Not decisive - One/multiple location(s) where experimental gene/protein characterization data is partly supporting and partly conflicting.
 

Comment

 

         Cell line U-2 OS

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Location(s)

 

Staining characteristics

 

Staining intensity

Negative 

Validation

No staining.
 

Comment

 

         Cell line U-251 MG

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Location(s)

Nucleus but not nucleoli, Cytoplasm 

Staining characteristics

 

Staining intensity

Weak 

Validation

Not decisive - One/multiple location(s) where experimental gene/protein characterization data is partly supporting and partly conflicting.
 

Comment

 

         Antibody summary


Immunofluorescent staining of human cell line A-431 shows positivity in nucleus but not nucleoli, cytoplasm & golgi apparatus.
 

Summary

Staining of cytoplasm and nucleus in A-431 and U-251 MG with additional staining of Golgi in A-431.  

Validation

The subcellular location is partly supported by literature or no literature is available.
 

Dictionary

Cytoplasm
Golgi apparatus
Nucleus but not nucleoli