ACTN1

ANTIBODY AND ANTIGEN INFORMATION

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Antibody HPA006035

 

Antibody CAB004303

 

ANTIBODY INFORMATION

Provider

Atlas Antibodies
Sigma-Aldrich
 Santa Cruz Biotechnology
 

Product name

HPA006035 sc-17829 

Host species

Rabbit Mouse 

Clonality

pAb mAb 

Purity

Affinity purified using the PrEST-antigen as affinity ligand Protein A/G 

Released in version

3 2 

ANTIGEN INFORMATION

Antigen

Recombinant protein fragment Recombinant protein 

Length (aa)

121  

Antigen sequence

SAKEGLLLWCQRKTAPYKNVNIQNFHISWKDGLGFCALIHRHRPELIDYG
KLRKDDPLTNLNTAFDVAEKYLDIPKMLDAEDIVGTARPDEKAIMTYVSS
FYHAFSGAQKAETAANRICKV
 
 

Matching transcripts

ACTN1-001 - ENSP00000193403 [100%]
ACTN1-002 - ENSP00000414272 [100%]
ACTN1-005 - ENSP00000377941 [100%]
ACTN1-006 - ENSP00000439828 [100%]
ACTN1-007 - ENSP00000366035 [100%]
ACTN1-011 - ENSP00000450764 [100%]
ACTN1-012 - ENSP00000450903 [100%]
  

Other gene match

ACTN2 - ENSG00000077522 [83%]
ACTN4 - ENSG00000130402 [93%]
ACTN3 - ENSG00000248746 [86%]
  

ANTIBODY VALIDATION

         Immunohistochemistry

Image

  

Description

Immunohistochemical staining of human smooth muscle shows cytoplasmic positivity in smooth muscle cells.
More information
 Application not done for this antibody. 

Retrieval method

HIER pH6  

Antibody dilution

1:350  

Literature conformity

Partly consistent with extensive gene/protein characterization data  

RNA consistency

Mainly consistent with RNA expression data  

         Immunofluorescence

Image

  

Description

Application not done for this antibody. Immunofluorescent staining of human cell line U-2 OS shows positivity in plasma membrane & focal adhesion sites.
More information
 

Antibody dilution

 1:50 

Validation IF

 Supportive: The subcellular location is supported by experimental gene/protein characterization data, gene silencing, or an independent antibody. 

         Western Blot

Image

  

Description

Lane 1: Marker [kDa] 229, 112, 83.5, 47.9, 32.3, 26.5, 17.2
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil
More information
 Lane 1: Marker [kDa] 230, 110, 82, 49.3, 32.2, 25.5, 17.6
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil
More information
 

Target mass (kDa)

107.6, 107.1, 105.6, 104.9, 103.9, 103.2, 103.1, 102.7, 94.8, 29.6, 27.1 107.1, 105.6, 103.1, 102.7, 94.8, 34.3, 29.6, 28.8, 27.1, 24.3, 16.3, 14.0, 9.4, 8.2 

Antibody dilution

1:250 1:500 

Validation WB

Supportive: Single band corresponding to the predicted size in kDa (+/-20%) Supportive: Single band corresponding to the predicted size in kDa (+/-20%) 

         Protein array

Image

  

Description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
More information
 Application not done for this antibody. 

Antibody dilution

1:2000  

Validation PA

Supportive: Pass with single peak corresponding to interaction only with its own antigen.  
 

ANTIGEN VIEW

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ACTN1-001
 
ACTN1-002
 
ACTN1-005
 
ACTN1-006
 
ACTN1-007
 
ACTN1-011
 
ACTN1-012