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SUBCELLULAR LOCATION

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Main location(s)

Cytoskeleton (Actin filaments)

Additional location(s)

 

Staining summary

Mainly localized to actin filaments.

Reliability (APE)

Low

Antibodies in assay

HPA006035, HPA008315
 

Antibody HPA006035

 

Antibody HPA008315

 

         Cell line A-431

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Location(s)

Cytoskeleton (Actin filaments)   

Staining characteristics

   

Staining intensity

Strong   

Validation

The multi-targeting antibody yielding a staining pattern consistent with available gene/protein characterization data for at least one of the genes but not all.
   

Comment

   

         Cell line U-2 OS

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Location(s)

Cytoskeleton (Actin filaments)  

Staining characteristics

  

Staining intensity

Moderate Negative 

Validation

The multi-targeting antibody yielding a staining pattern consistent with available gene/protein characterization data for at least one of the genes but not all.
 
No staining.
 

Comment

  

         Cell line U-251 MG

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Location(s)

Nucleoli, Cytoskeleton (Actin filaments)  

Staining characteristics

  

Staining intensity

Moderate Negative 

Validation

The multi-targeting antibody yielding a staining pattern consistent with available gene/protein characterization data for at least one of the genes but not all.
 
No staining.
 

Comment

  

         Antibody summary


Immunofluorescent staining of human cell line A-431 shows positivity in cytoskeleton (actin filaments).
 

Immunofluorescent staining of human cell line U-251 MG shows no positivity.
 

Summary

Staining of cytoskeleton (actin filaments) in all three cell lines with additional staining of nucleoli in U-251 MG.   No staining was observed in any of the three cell lines.  

Validation

The subcellular location is partly supported by literature or no literature is available.
 
The subcellular location is partly supported by literature or no literature is available.
 

Dictionary

Actin filaments