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SUBCELLULAR LOCATION

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Main location(s)

Plasma membrane, Cytoplasm

Additional location(s)

 

Staining summary

Mainly localized to the plasma membrane and cytoplasm.

Reliability (APE)

Medium

Antibodies in assay

HPA019513, HPA019523
 

Antibody HPA019513

 

Antibody HPA019523

 

         Cell line A-431

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Location(s)

Nuclear membrane, Cytoplasm Nucleoli, Plasma membrane, Cytoplasm, Centrosome 

Staining characteristics

  

Staining intensity

Weak Strong 

Validation

Not decisive - One/multiple location(s) where experimental gene/protein characterization data is partly supporting and partly conflicting.
 
One/multiple location(s) with no available experimental gene/protein characterization data.
 

Comment

  

         Cell line U-2 OS

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Location(s)

Plasma membrane, Cytoplasm Plasma membrane, Cytoplasm 

Staining characteristics

  

Staining intensity

Moderate Strong 

Validation

Not decisive - One/multiple location(s) where experimental gene/protein characterization data is partly supporting and partly conflicting.
 
One/multiple location(s) with no available experimental gene/protein characterization data.
 

Comment

  

         Cell line U-251 MG

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Location(s)

Plasma membrane, Focal Adhesions Plasma membrane, Cytoplasm 

Staining characteristics

  

Staining intensity

Moderate Strong 

Validation

Not decisive - One/multiple location(s) where experimental gene/protein characterization data is partly supporting and partly conflicting.
 
One/multiple location(s) with no available experimental gene/protein characterization data.
 

Comment

  

         Antibody summary


Immunofluorescent staining of human cell line U-251MG shows positivity in plasma membrane & focal adhesions.
 

Immunofluorescent staining of human cell line A-431 shows positivity in nucleoli, plasma membrane, cytoplasm & centrosome.
 

Summary

Staining of plasma membrane and focal adhesions in U-251 MG. Staining of plasma membrane and cytoplasm in U-2 OS. Staining of cytoplasm and nuclear membrane in A-431.   Staining of cytoplasm and plasma membrane in all three cell lines with additional staining of nucleoli and centrosome in A-431.  

Validation

The subcellular location is partly supported by literature or no literature is available.
 
The subcellular location is partly supported by literature or no literature is available.
 

Dictionary

Cytoplasm
Plasma membrane