AGBL5

ANTIBODY AND ANTIGEN INFORMATION

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Antibody HPA030981

 

ANTIBODY INFORMATION

Provider

Atlas Antibodies
Sigma-Aldrich
 

Product name

HPA030981 

Host species

Rabbit 

Clonality

pAb 

Purity

Affinity purified using the PrEST-antigen as affinity ligand 

Released in version

6 

ANTIGEN INFORMATION

Antigen

Recombinant protein fragment 

Length (aa)

86 

Antigen sequence

TTFFAFCYPFSYSDCQELLNQLDQRFPENHPTHSSPLDTIYYRRELLCYS
LDGLRVDLLTITSCHGLREDREPRLEQLFPDTSTPR
 

Matching transcripts

AGBL5-001 - ENSP00000433830 [99%]
AGBL5-004 - ENSP00000353249 [99%]
AGBL5-005 - ENSP00000323681 [99%]
 

Other gene match

 

ANTIBODY VALIDATION

         Immunohistochemistry

Image

 

Description

Immunohistochemical staining of human testis shows moderate cytoplasmic positivity in cells in seminiferous ducts and Leydig cells.
More information
 

Retrieval method

HIER pH6 

Antibody dilution

1:100 

Literature conformity

Partly consistent with gene/protein characterization data 

RNA consistency

Mainly not consistent with RNAseq data 

         Immunofluorescence

Image

 

Description

Immunofluorescent staining of human cell line U-251 MG shows positivity in cytoplasm.
More information
 

Antibody dilution

1:106 

Validation IF

Uncertain: The subcellular location is partly supported by literature or no literature is available. 

         Western Blot

Image

 

Description

 

Target mass (kDa)

97.5, 90.3, 80.0 

Antibody dilution

1:250 

Validation WB

Non-supportive: Weak band of predicted size but with additional bands of higher intensity also present 

         Protein array

Image

 

Description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
More information
 

Antibody dilution

1:3000 

Validation PA

Supportive: Pass with single peak corresponding to interaction only with its own antigen. 
 

ANTIGEN VIEW

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AGBL5-001
 
AGBL5-004
 
AGBL5-005