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SUBCELLULAR LOCATION

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Main location(s)

Plasma membrane, Cytoplasm

Additional location(s)

 

Staining summary

Localized to the cytoplasm and plasma membrane. .

Reliability (APE)

Medium

Antibodies in assay

CAB003681, HPA021241, HPA024031
 

Antibody CAB003681

 

Antibody HPA021241

 

Antibody HPA024031

 

         Cell line A-431

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Location(s)

Cytoplasm Nucleus but not nucleoli, Plasma membrane, Cytoplasm  

Staining characteristics

   

Staining intensity

Weak Moderate Negative 

Validation

One/multiple location(s) with no available experimental gene/protein characterization data.
 
One/multiple location(s) with no available experimental gene/protein characterization data.
 
No staining.
 

Comment

   

         Cell line U-2 OS

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Location(s)

Cytoplasm Plasma membrane, Cytoplasm Plasma membrane, Cytoplasm 

Staining characteristics

 Smooth  

Staining intensity

Weak Moderate Moderate 

Validation

One/multiple location(s) with no available experimental gene/protein characterization data.
 
One/multiple location(s) with no available experimental gene/protein characterization data.
 
One/multiple location(s) with no available experimental gene/protein characterization data.
 

Comment

   

         Cell line U-251 MG

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Location(s)

Cytoplasm Plasma membrane, Cytoplasm  

Staining characteristics

 Smooth  

Staining intensity

Weak Moderate Negative 

Validation

One/multiple location(s) with no available experimental gene/protein characterization data.
 
One/multiple location(s) with no available experimental gene/protein characterization data.
 
No staining.
 

Comment

   

         Antibody summary


Immunofluorescent staining of human cell line U-2 OS shows positivity in cytoplasm.
 

Immunofluorescent staining of human cell line U-2 OS shows positivity in plasma membrane & cytoplasm.
 

Immunofluorescent staining of human cell line U-2 OS shows positivity in plasma membrane & cytoplasm.
 

Summary

Staining of cytoplasm in all three cell lines.   Staining of cytoplasm and plasma membrane in all three cell lines with additional staining of nucleus in A-431.   Staining of cytoplasm and plasma membrane in U-2 OS.  

Validation

The subcellular location is partly supported by literature or no literature is available.
 
The subcellular location is partly supported by literature or no literature is available.
 
The subcellular location is partly supported by literature or no literature is available.
 

Dictionary

Cytoplasm
Plasma membrane