CYB5R3

ANTIBODY AND ANTIGEN INFORMATION

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Antibody HPA001566

 

ANTIBODY INFORMATION

Provider

Atlas Antibodies
Sigma-Aldrich
 

Product name

HPA001566 

Host species

Rabbit 

Clonality

pAb 

Purity

Affinity purified using the PrEST-antigen as affinity ligand 

Released in version

2 

ANTIGEN INFORMATION

Antigen

Recombinant protein fragment 

Length (aa)

145 

Antigen sequence

SGLLVYQGKGKFAIRPDKKSNPIIRTVKSVGMIAGGTGITPMLQVIRAIM
KDPDDHTVCHLLFANQTEKDILLRPELEELRNKHSARFKLWYTLDRAPEA
WDYGQGFVNEEMIRDHLPPPEEEPLVLMCGPPPMIQYACLPNLDH
 

Matching transcripts

CYB5R3-001 - ENSP00000338461 [100%]
CYB5R3-002 - ENSP00000384834 [100%]
CYB5R3-003 - ENSP00000384457 [100%]
CYB5R3-007 - ENSP00000385679 [100%]
CYB5R3-201 - ENSP00000354468 [100%]
CYB5R3-202 - ENSP00000379597 [100%]
 

Other gene match

 

ANTIBODY VALIDATION

         Immunohistochemistry

Image

 

Description

Immunohistochemical staining of human testis shows strong cytoplasmic positivity in cells in seminiferus ducts and Leydig cells.
More information
 

Retrieval method

HIER pH6 

Antibody dilution

1:1500 

Validation IH

Supportive: One antibody yielding a staining pattern which is consistent with gene/protein characterization data 

         Immunofluorescence

Image

 

Description

Immunofluorescent staining of human cell line A-431 shows positivity in endoplasmic reticulum.
More information
 

Antibody dilution

1:83 

Validation IF

Supportive: The subcellular location is supported by literature. 

         Western Blot

Image

 

Description

Lane 1: Marker [kDa] 206, 113, 82, 49, 32, 26, 17.8
Lane 2: RT-4
Lane 3: U-251 MG
Lane 4: A-431
Lane 5: Liver
Lane 6: Tonsil
More information
 

Target mass (kDa)

38.2, 34.2, 31.6 

Antibody dilution

1:250 

Validation WB

Supportive: Single band corresponding to the predicted size in kDa (+/-20%) 

         Protein array

Image

 

Description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
More information
 

Antibody dilution

1:3000 

Validation PA

Supportive: Pass with single peak corresponding to interaction only with its own antigen. 
 

ANTIGEN VIEW

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CYB5R3-001
 
CYB5R3-002
 
CYB5R3-003
 
CYB5R3-007
 
CYB5R3-201
 
CYB5R3-202