Fields »

SUBCELLULAR LOCATION

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Main location(s)

Cytoplasm

Additional location(s)

Centrosome 

Staining summary

Mainly localized to cytoplasm. In addition localized to centrosome.

Reliability (APE)

High

Antibodies in assay

HPA003600, CAB019391
 

Antibody HPA003600

 

Antibody CAB019391

 

         Cell line A-431

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Location(s)

Cytoplasm, Centrosome Cytoplasm 

Staining characteristics

Smooth  

Staining intensity

Strong Moderate 

Validation

One/multiple location(s) supported by experimental gene/protein characterization data.
 
One/multiple location(s) supported by experimental gene/protein characterization data.
 

Comment

  

         Cell line U-2 OS

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Location(s)

Cytoplasm Cytoplasm 

Staining characteristics

Smooth  

Staining intensity

Strong Moderate 

Validation

One/multiple location(s) supported by experimental gene/protein characterization data.
 
One/multiple location(s) supported by experimental gene/protein characterization data.
 

Comment

  

         Cell line U-251 MG

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Location(s)

Cytoplasm, Centrosome Cytoplasm 

Staining characteristics

Smooth  

Staining intensity

Moderate Weak 

Validation

One/multiple location(s) supported by experimental gene/protein characterization data.
 
One/multiple location(s) supported by experimental gene/protein characterization data.
 

Comment

  

         Antibody summary


Immunofluorescent staining of human cell line U-2 OS shows positivity in cytoplasm.
 

Immunofluorescent staining of human cell line U-251 MG shows positivity in cytoplasm.
 

Summary

Staining of cytoplasm in all three cell lines with additional staining of centrosomes in U-251 MG and A-431.   Staining of cytoplasm in all three cell lines.  

Validation

The subcellular location is supported by literature.
 
The subcellular location is supported by literature.
 

Dictionary

Centrosome
Cytoplasm