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SUBCELLULAR LOCATION

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Main location(s)

Plasma membrane, Vesicles

Additional location(s)

Nuclear membrane 

Staining summary

Staining of vesicles and plasma membrane in all three cell lines with additional staining of nuclear membrane in U-2 OS.

Reliability (Single)

Antibodies in assay

HPA019100
 

Antibody HPA019100

 

         Cell line A-431

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Location(s)

Plasma membrane, Vesicles 

Staining characteristics

 

Staining intensity

Moderate 

Validation

One/multiple location(s) in cytoplasm (e.g. golgi, mitochondria) supported by experimental evidence for cytoplasmic localization.
 

Comment

 

         Cell line U-2 OS

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Location(s)

Nuclear membrane, Plasma membrane, Vesicles 

Staining characteristics

 

Staining intensity

Moderate 

Validation

One/multiple location(s) in cytoplasm (e.g. golgi, mitochondria) supported by experimental evidence for cytoplasmic localization.
 

Comment

 

         Cell line U-251 MG

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Location(s)

Plasma membrane, Vesicles 

Staining characteristics

 

Staining intensity

Moderate 

Validation

One/multiple location(s) in cytoplasm (e.g. golgi, mitochondria) supported by experimental evidence for cytoplasmic localization.
 

Comment

 

         Antibody summary


Immunofluorescent staining of human cell line U-2 OS shows positivity in nuclear membrane, plasma membrane & vesicles.
 

Summary

Staining of vesicles and plasma membrane in all three cell lines with additional staining of nuclear membrane in U-2 OS.  

Validation

The subcellular location is supported by literature.
 

Dictionary

Nuclear membrane
Plasma membrane
Endosome