Fields »

SUBCELLULAR LOCATION

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Main location(s)

Nucleus but not nucleoli, Plasma membrane

Additional location(s)

 

Staining summary

Localized to the nucleus or the plasma membrane.

Reliability (APE)

Very low

Antibodies in assay

HPA007419, CAB007754
 

Antibody HPA007419

 

Antibody CAB007754

 

         Cell line A-431

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Location(s)

Nucleus but not nucleoli, Cytoplasm Plasma membrane 

Staining characteristics

  

Staining intensity

Weak Weak 

Validation

One/multiple location(s) with no available experimental gene/protein characterization data or partly supported and partly conflicted, but showing dissimilar staining to ≥1 other antibody.
 
One/multiple location(s) with no available experimental gene/protein characterization data or partly supported and partly conflicted, but showing dissimilar staining to ≥1 other antibody.
 

Comment

  

         Cell line U-2 OS

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Location(s)

Nucleus but not nucleoli  

Staining characteristics

Granular  

Staining intensity

Weak Negative 

Validation

One/multiple location(s) with no available experimental gene/protein characterization data or partly supported and partly conflicted, but showing dissimilar staining to ≥1 other antibody.
 
No staining.
 

Comment

  

         Cell line U-251 MG

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Location(s)

Nucleus but not nucleoli   

Staining characteristics

Granular   

Staining intensity

Weak   

Validation

One/multiple location(s) with no available experimental gene/protein characterization data or partly supported and partly conflicted, but showing dissimilar staining to ≥1 other antibody.
   

Comment

   

         Antibody summary


Immunofluorescent staining of human cell line U-2 OS shows positivity in nucleus but not nucleoli.
 

Immunofluorescent staining of human cell line A-431 shows positivity in plasma membrane.
 

Summary

Staining of nucleus in all three cell lines with additional staining of cytoplasm in A-431.   Staining of plasma membrane in A-431.  

Validation

The subcellular location is partly supported by literature or no literature is available.
 
The subcellular location is partly supported by literature or no literature is available.
 

Dictionary

Nucleus but not nucleoli
Plasma membrane