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SUBCELLULAR LOCATION

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Main location(s)

Nucleus, Nucleus but not nucleoli, Endoplasmic reticulum

Additional location(s)

Cytoplasm 

Staining summary

Mainly localized to the nucleus but also to the cytoplasm. Could also be localized to the endoplasmic reticulum.

Reliability (APE)

High

Antibodies in assay

CAB005072, HPA029722, HPA029723
 

Antibody CAB005072

 

Antibody HPA029722

 

Antibody HPA029723

 

         Cell line A-431

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Location(s)

Endoplasmic reticulum Nucleus but not nucleoli Nucleus but not nucleoli 

Staining characteristics

  Smooth 

Staining intensity

Strong Weak Strong 

Validation

One/multiple location(s) in cytoplasm (e.g. golgi, mitochondria) supported by experimental evidence for cytoplasmic localization.
 
One/multiple location(s) supported by experimental gene/protein characterization data and ≥1 other antibody.
 
One/multiple location(s) supported by experimental gene/protein characterization data and ≥1 other antibody.
 

Comment

   

         Cell line U-2 OS

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Location(s)

Endoplasmic reticulum   Nucleus but not nucleoli, Cytoplasm 

Staining characteristics

    

Staining intensity

Moderate   Strong 

Validation

One/multiple location(s) in cytoplasm (e.g. golgi, mitochondria) supported by experimental evidence for cytoplasmic localization.
   
One/multiple location(s) supported by experimental gene/protein characterization data and ≥1 other antibody.
 

Comment

    

         Cell line U-251 MG

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Location(s)

Nucleus but not nucleoli, Endoplasmic reticulum Nucleus but not nucleoli, Cytoplasm Nucleus but not nucleoli 

Staining characteristics

  Smooth 

Staining intensity

Strong Weak Strong 

Validation

Multiple locations partly supported (at least one) by experimental gene/protein characterization data.
 
One/multiple location(s) supported by experimental gene/protein characterization data and ≥1 other antibody.
 
One/multiple location(s) supported by experimental gene/protein characterization data and ≥1 other antibody.
 

Comment

   

         Antibody summary


Immunofluorescent staining of human cell line U-251 MG shows positivity in nucleus but not nucleoli & endoplasmic reticulum.
 

Immunofluorescent staining of human cell line U-251MG shows positivity in nucleus but not nucleoli & cytoplasm.
 

Immunofluorescent staining of human cell line A-431 shows positivity in nucleus but not nucleoli.
 

Summary

Staining of endoplasmic reticulum in all three cell lines, with additional staining of nucleus in U-251 MG.   Staining of nucleus in A-431 and U-251 MG with additional staining of cytoplasm in U-251 MG.   Staining of nucleus in all thre cell lines with additional staining of cytoplasm in U-251 MG and U-2 OS.  

Validation

The subcellular location is supported by literature.
 
The subcellular location is supported by literature.
 
The subcellular location is supported by literature.
 

Dictionary

Cytoplasm
Endoplasmic reticulum
Nucleus
Nucleus but not nucleoli