KCNT1

ANTIBODY AND ANTIGEN INFORMATION

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Antibody HPA059880

 

ANTIBODY INFORMATION

Provider

Atlas Antibodies
Sigma-Aldrich
 

Product name

HPA059880 

Host species

Rabbit 

Clonality

pAb 

Purity

Affinity purified using the PrEST-antigen as affinity ligand 

Released in version

12 

ANTIGEN INFORMATION

Antigen

Recombinant protein fragment 

Length (aa)

32 

Antigen sequence

LAHVASSSQSRKSSCSHKLSSCNPETRDETQL
 

Matching transcripts

KCNT1-001 - ENSP00000417851 [100%]
KCNT1-002 - ENSP00000360822 [100%]
KCNT1-004 - ENSP00000419086 [100%]
KCNT1-005 - ENSP00000419007 [100%]
KCNT1-006 - ENSP00000418003 [100%]
KCNT1-007 - ENSP00000417578 [100%]
KCNT1-201 - ENSP00000263604 [100%]
KCNT1-202 - ENSP00000298480 [100%]
 

Other gene match

 

ANTIBODY VALIDATION

         Immunohistochemistry

Image

 

Description

Immunohistochemical staining of human stomach, upper shows strong membranous positivity in glandular cells.
More information
 

Retrieval method

HIER pH6 

Antibody dilution

1:250 

Literature conformity

No avaliable characterization data 

RNA consistency

Not at all consistent with RNAseq data 

         Western Blot

Image

 

Description

Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil
More information
 

Target mass (kDa)

142.0, 139.7, 138.9, 138.3, 137.6, 137.4, 137.0 

Antibody dilution

1:130 

Validation WB

Uncertain: Single band differing more than +/-20% from predicted size in kDa and not supported by experimental and/or bioinformatic data 

         Protein array

Image

 

Description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
More information
 

Antibody dilution

1:5000 

Validation PA

Supportive: Pass with single peak corresponding to interaction only with its own antigen. 
 

ANTIGEN VIEW

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KCNT1-001
 
KCNT1-002
 
KCNT1-004
 
KCNT1-005
 
KCNT1-006
 
KCNT1-007
 
KCNT1-201
 
KCNT1-202