Fields »

SUBCELLULAR LOCATION

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Main location(s)

Nucleus

Additional location(s)

 

Staining summary

Localized to the nucleus.

Reliability (APE)

High

Antibodies in assay

HPA037924, HPA037925
 

Antibody HPA037924

 

Antibody HPA037925

 

         Cell line A-431

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Location(s)

Nucleus Nucleus but not nucleoli, Vesicles, Cytoskeleton (Intermediate filaments) 

Staining characteristics

  

Staining intensity

Moderate Moderate 

Validation

One/multiple location(s) supported by experimental gene/protein characterization data.
 
Multiple locations partly supported (at least one) by experimental gene/protein characterization data.
 

Comment

  

         Cell line U-2 OS

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Location(s)

 Nucleus but not nucleoli 

Staining characteristics

  

Staining intensity

Negative Weak 

Validation

No staining.
 
One/multiple location(s) supported by experimental gene/protein characterization data.
 

Comment

  

         Cell line U-251 MG

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Location(s)

Nucleus, Nucleoli Nucleus but not nucleoli 

Staining characteristics

Spotty  

Staining intensity

Weak Weak 

Validation

One/multiple location(s) supported by experimental gene/protein characterization data.
 
One/multiple location(s) supported by experimental gene/protein characterization data.
 

Comment

  

         Antibody summary


Immunofluorescent staining of human cell line U-251 MG shows positivity in nucleus & nucleoli.
 

Immunofluorescent staining of human cell line U-251 MG shows positivity in nucleus but not nucleoli.
 

Summary

Staining of nucleus in A-431 and nucleus and nucleoli in U-251 MG.   Staining of nucleus in all three cell lines with additional staining of vesicles and cytoskeleton (intermediate filaments) in A-431.  

Validation

The subcellular location is supported by literature.
 
The subcellular location is supported by literature.
 

Dictionary

Nucleus