Fields »

SUBCELLULAR LOCATION

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Main location(s)

Cytoplasm

Additional location(s)

Nucleoli, Mitochondria, Golgi apparatus, Vesicles 

Staining summary

Mainly localized to cytoplasm. Additionally localized to mitochondria, nucleoli, vesicles and Golgi apparatus.

Reliability (APE)

Low

Antibodies in assay

HPA022141, HPA022882, HPA027883
 

Antibody HPA022141

 

Antibody HPA022882

 

Antibody HPA027883

 

         Cell line A-431

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Location(s)

Cytoplasm Golgi apparatus Mitochondria 

Staining characteristics

Granular   

Staining intensity

Weak Moderate Strong 

Validation

One/multiple location(s) with no available experimental gene/protein characterization data or partly supported and partly conflicted, but showing dissimilar staining to ≥1 other antibody.
 
One/multiple location(s) with no available experimental gene/protein characterization data or partly supported and partly conflicted, but showing dissimilar staining to ≥1 other antibody.
 
One/multiple location(s) with no available experimental gene/protein characterization data or partly supported and partly conflicted, but showing dissimilar staining to ≥1 other antibody.
 

Comment

   

         Cell line U-2 OS

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Location(s)

Nucleoli, Cytoplasm Cytoplasm Cytoplasm 

Staining characteristics

Granular, Fibrous Granular Granular 

Staining intensity

Weak Weak Moderate 

Validation

One/multiple location(s) with no available experimental gene/protein characterization data or partly supported and partly conflicted, but showing dissimilar staining to ≥1 other antibody.
 
One/multiple location(s) with no available experimental gene/protein characterization data or partly supported and partly conflicted, but showing dissimilar staining to ≥1 other antibody.
 
One/multiple location(s) with no available experimental gene/protein characterization data or partly supported and partly conflicted, but showing dissimilar staining to ≥1 other antibody.
 

Comment

   

         Cell line U-251 MG

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Location(s)

Nucleoli, Cytoplasm Vesicles Cytoplasm 

Staining characteristics

  Granular 

Staining intensity

Moderate Moderate Strong 

Validation

One/multiple location(s) with no available experimental gene/protein characterization data or partly supported and partly conflicted, but showing dissimilar staining to ≥1 other antibody.
 
One/multiple location(s) with no available experimental gene/protein characterization data or partly supported and partly conflicted, but showing dissimilar staining to ≥1 other antibody.
 
One/multiple location(s) with no available experimental gene/protein characterization data or partly supported and partly conflicted, but showing dissimilar staining to ≥1 other antibody.
 

Comment

   

         Antibody summary


Immunofluorescent staining of human cell line U-2 OS shows positivity in nucleoli & cytoplasm.
 

Immunofluorescent staining of human cell line U-251MG shows positivity in vesicles.
 

Immunofluorescent staining of human cell line A-431 shows positivity in mitochondria.
 

Summary

Staining of nucleoli and cytoplasm in U-251 MG and U-2 OS. Staining of cytoplasm in A-431.   Staining of cytoplasm in U-2 OS, vesicles in U-251 MG and Golgi in A-431.   Staining of cytoplasm in U-251 MG and U-2 OS. Staining of mitochondria in a fraction of the A-431 cells.  

Validation

The subcellular location is partly supported by literature or no literature is available.
 
The subcellular location is partly supported by literature or no literature is available.
 
The subcellular location is partly supported by literature or no literature is available.
 

Dictionary

Cytoplasm
Golgi apparatus
Mitochondria
Nucleoli
Endosome