MPP2

ANTIBODY AND ANTIGEN INFORMATION

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Antibody HPA026486

 

ANTIBODY INFORMATION

Provider

Atlas Antibodies
Sigma-Aldrich
 

Product name

HPA026486 

Host species

Rabbit 

Clonality

pAb 

Purity

Affinity purified using the PrEST-antigen as affinity ligand 

Released in version

5 

ANTIGEN INFORMATION

Antigen

Recombinant protein fragment 

Length (aa)

59 

Antigen sequence

LRAMNRAALESGISTKQLTEADLRRTVEESSRIQRGYGHYFDLCLVNSNL
ERTFRELQT
 

Matching transcripts

MPP2-001 - ENSP00000269095 [100%]
MPP2-002 - ENSP00000366389 [100%]
MPP2-003 - ENSP00000428286 [100%]
MPP2-005 - ENSP00000428182 [100%]
MPP2-007 - ENSP00000430540 [100%]
MPP2-008 - ENSP00000428136 [100%]
MPP2-201 - ENSP00000438012 [100%]
 

Other gene match

 

ANTIBODY VALIDATION

         Immunohistochemistry

Image

 

Description

Immunohistochemical staining of human cerebral cortex shows moderate cytoplasmic positivity in neuronal cells.
More information
 

Retrieval method

HIER pH6 

Antibody dilution

1:10 

Validation IH

Uncertain: One antibody yielding a staining pattern and there is no available gene/protein characterization data or the staining pattern is partly consistent with or contradicted by limited gene/protein characterization data 

         Western Blot

Image

 

Description

Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: Negative control (vector only transfected HEK293T lysate)
Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY417352)
More information
 

Target mass (kDa)

66.8, 64.6, 63.3, 61.6, 60.5, 46.5 

Antibody dilution

1:250 

Validation WB

Supportive: Single band corresponding to the predicted size in kDa (+/-20%) 

         Protein array

Image

 

Description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
More information
 

Antibody dilution

1:500 

Validation PA

Supportive: Pass with single peak corresponding to interaction only with its own antigen. 
 

ANTIGEN VIEW

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MPP2-001
 
MPP2-002
 
MPP2-003
 
MPP2-005
 
MPP2-007
 
MPP2-008
 
MPP2-201