Fields »

SUBCELLULAR LOCATION

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Main location(s)

Nucleus but not nucleoli, Vesicles

Additional location(s)

 

Staining summary

Localized to the nucleus and vesicles.

Reliability (APE)

Medium

Antibodies in assay

HPA018666, HPA018900
 

Antibody HPA018666

 

Antibody HPA018900

 

         Cell line A-431

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Location(s)

Nucleus but not nucleoli, Vesicles Nucleus but not nucleoli, Vesicles 

Staining characteristics

  

Staining intensity

Weak Moderate 

Validation

Location not consistent with experimental gene/protein characterization data, but supported by ≥1 other antibody.
 
Location not consistent with experimental gene/protein characterization data, but supported by ≥1 other antibody.
 

Comment

  

         Cell line U-2 OS

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Location(s)

Nucleus but not nucleoli, Vesicles Nucleus but not nucleoli, Vesicles 

Staining characteristics

  

Staining intensity

Moderate Moderate 

Validation

Location not consistent with experimental gene/protein characterization data, but supported by ≥1 other antibody.
 
Location not consistent with experimental gene/protein characterization data, but supported by ≥1 other antibody.
 

Comment

  

         Cell line U-251 MG

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Location(s)

Nucleus but not nucleoli, Vesicles Nucleus but not nucleoli, Vesicles 

Staining characteristics

Granular  

Staining intensity

Moderate Moderate 

Validation

Location not consistent with experimental gene/protein characterization data, but supported by ≥1 other antibody.
 
Location not consistent with experimental gene/protein characterization data, but supported by ≥1 other antibody.
 

Comment

  

         Antibody summary


Immunofluorescent staining of human cell line U-251MG shows positivity in nucleus but not nucleoli & vesicles.
 

Immunofluorescent staining of human cell line U-2 OS shows positivity in nucleus but not nucleoli & vesicles.
 

Summary

Staining of nucleus and vesicles in all three cell lines.   Staining of nucleus and vesicles in all three cell lines.  

Validation

The subcellular location is partly supported by literature or no literature is available.
 
The subcellular location is partly supported by literature or no literature is available.
 

Dictionary

Nucleus but not nucleoli
Endosome