Fields »

SUBCELLULAR LOCATION

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Main location(s)

Cytoplasm

Additional location(s)

Nucleus but not nucleoli, Plasma membrane 

Staining summary

Mainly localized to cytoplasm but also to nucleus and plasma membrane.

Reliability (APE)

Low

Antibodies in assay

HPA001536, CAB035996
 

Antibody HPA001536

 

Antibody CAB035996

 

         Cell line A-431

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Location(s)

Nucleus but not nucleoli Plasma membrane, Cytoplasm 

Staining characteristics

  

Staining intensity

Weak Moderate 

Validation

One/multiple location(s) with no available experimental gene/protein characterization data.
 
One/multiple location(s) with no available experimental gene/protein characterization data.
 

Comment

  

         Cell line U-2 OS

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Location(s)

Nucleus but not nucleoli, Cytoplasm Plasma membrane, Cytoplasm 

Staining characteristics

Granular  

Staining intensity

Weak Moderate 

Validation

One/multiple location(s) with no available experimental gene/protein characterization data.
 
One/multiple location(s) with no available experimental gene/protein characterization data.
 

Comment

  

         Cell line U-251 MG

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Location(s)

Nucleus but not nucleoli, Cytoplasm Plasma membrane, Cytoplasm 

Staining characteristics

  

Staining intensity

Weak Moderate 

Validation

One/multiple location(s) with no available experimental gene/protein characterization data.
 
One/multiple location(s) with no available experimental gene/protein characterization data.
 

Comment

  

         Antibody summary


Immunofluorescent staining of human cell line U-251MG shows positivity in nucleus but not nucleoli & cytoplasm.
 

Immunofluorescent staining of human cell line U-251MG shows positivity in plasma membrane & cytoplasm.
 

Summary

Staining of nuclei but not nucleoli in all three cell lines. Additional granular staining of cytoplasm in U-251MG and U-2 OS.   Staining of cytoplasm and plasma membrane in all three cell lines with additional staining of endoplasmic reticulum in A-431.  

Validation

The subcellular location is partly supported by literature or no literature is available.
 
The subcellular location is partly supported by literature or no literature is available.
 

Dictionary

Cytoplasm
Nucleus but not nucleoli
Plasma membrane