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SUBCELLULAR LOCATION

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Main location(s)

Plasma membrane, Cytoplasm

Additional location(s)

Nucleus but not nucleoli, Cytoskeleton (Actin filaments) 

Staining summary

Staining of plasma membrane and cytoplasm in all three cell lines with additional staining of nucleus and cytoskeleton (actin filaments) in U-2 OS.

Reliability (Single)

Antibodies in assay

HPA017286
 

Antibody HPA017286

 

         Cell line A-431

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Location(s)

Plasma membrane, Cytoplasm 

Staining characteristics

Granular 

Staining intensity

Weak 

Validation

One/multiple location(s) with no available experimental gene/protein characterization data.
 

Comment

 

         Cell line U-2 OS

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Location(s)

Nucleus but not nucleoli, Plasma membrane, Cytoplasm, Cytoskeleton (Actin filaments) 

Staining characteristics

 

Staining intensity

Weak 

Validation

One/multiple location(s) with no available experimental gene/protein characterization data.
 

Comment

 

         Cell line U-251 MG

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Location(s)

Plasma membrane, Cytoplasm 

Staining characteristics

 

Staining intensity

Weak 

Validation

One/multiple location(s) with no available experimental gene/protein characterization data.
 

Comment

 

         Antibody summary


Immunofluorescent staining of human cell line A-431 shows positivity in plasma membrane & cytoplasm.
 

Summary

Staining of plasma membrane and cytoplasm in all three cell lines with additional staining of nucleus and cytoskeleton (actin filaments) in U-2 OS.  

Validation

The subcellular location is partly supported by literature or no literature is available.
 

Dictionary

Cytoplasm
Actin filaments
Nucleus but not nucleoli
Plasma membrane