Fields »

SUBCELLULAR LOCATION

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Main location(s)

Plasma membrane, Cytoplasm

Additional location(s)

 

Staining summary

Mainly localized to the plasma membrane and cytoplasm.

Reliability (APE)

Medium

Antibodies in assay

HPA017931, HPA020289
 

Antibody HPA017931

 

Antibody HPA020289

 

         Cell line A-431

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Location(s)

Cytoplasm Plasma membrane, Cytoplasm 

Staining characteristics

Granular  

Staining intensity

Weak Moderate 

Validation

One/multiple location(s) with no available experimental gene/protein characterization data.
 
One/multiple location(s) with no available experimental gene/protein characterization data.
 

Comment

  

         Cell line U-2 OS

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Location(s)

Cytoplasm Plasma membrane, Cytoplasm 

Staining characteristics

  

Staining intensity

Moderate Moderate 

Validation

One/multiple location(s) with no available experimental gene/protein characterization data.
 
One/multiple location(s) with no available experimental gene/protein characterization data.
 

Comment

  

         Cell line U-251 MG

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Location(s)

Plasma membrane Plasma membrane, Cytoplasm, Mitochondria 

Staining characteristics

  

Staining intensity

Weak Moderate 

Validation

One/multiple location(s) with no available experimental gene/protein characterization data.
 
One/multiple location(s) with no available experimental gene/protein characterization data.
 

Comment

  

         Antibody summary


Immunofluorescent staining of human cell line U-251MG shows positivity in plasma membrane.
 

Immunofluorescent staining of human cell line U-2 OS shows positivity in plasma membrane & cytoplasm.
 

Summary

Staining of plasma membrane in U-251 MG and cytoplasm in A-431 and U-2 OS.   Staining of plasma membrane and cytoplasm in all three cell lines with additional staining of mitochondria in U-251 MG.  

Validation

The subcellular location is partly supported by literature or no literature is available.
 
The subcellular location is partly supported by literature or no literature is available.
 

Dictionary

Cytoplasm
Plasma membrane