Fields »

SUBCELLULAR LOCATION

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Main location(s)

Nucleus, Cytoplasm

Additional location(s)

 

Staining summary

Localized to the nucleus and cytoplasm.

Reliability (APE)

Medium

Antibodies in assay

HPA021591, HPA023300
 

Antibody HPA021591

 

Antibody HPA023300

 

         Cell line A-431

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Location(s)

 Nucleus but not nucleoli, Cytoplasm 

Staining characteristics

  

Staining intensity

Negative Moderate 

Validation

No staining.
 
Multiple locations partly supported (at least one) by experimental gene/protein characterization data.
 

Comment

  

         Cell line U-2 OS

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Location(s)

 Nucleus, Cytoplasm 

Staining characteristics

  

Staining intensity

Negative Moderate 

Validation

No staining.
 
Multiple locations partly supported (at least one) by experimental gene/protein characterization data.
 

Comment

  

         Cell line U-251 MG

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Location(s)

 Nucleus but not nucleoli, Cytoplasm 

Staining characteristics

 Spotty 

Staining intensity

Negative Moderate 

Validation

No staining.
 
Multiple locations partly supported (at least one) by experimental gene/protein characterization data.
 

Comment

  

         Antibody summary


Immunofluorescent staining of human cell line U-2 OS shows no positivity.
 

Immunofluorescent staining of human cell line U-2 OS shows positivity in nucleus & cytoplasm.
 

Summary

No staining was observed in any of the three cell lines.   Staining of nucleus and cytoplasm in all three cell lines.  

Validation

The subcellular location is partly supported by literature or no literature is available.
 
The subcellular location is supported by literature.
 

Dictionary

Cytoplasm
Nucleus