Fields »

SUBCELLULAR LOCATION

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Main location(s)

Mitochondria

Additional location(s)

Nucleus but not nucleoli, Cytoplasm 

Staining summary

Mainly localized to mitochondria.

Reliability (APE)

Medium

Antibodies in assay

HPA026506, HPA026528
 

Antibody HPA026506

 

Antibody HPA026528

 

         Cell line A-431

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Location(s)

Cytoplasm Nucleus but not nucleoli, Mitochondria 

Staining characteristics

  

Staining intensity

Strong Moderate 

Validation

Location not consistent with experimental gene/protein characterization data.
 
Multiple locations partly supported (at least one) by experimental gene/protein characterization data.
 

Comment

  

         Cell line U-2 OS

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Location(s)

Cytoplasm, Mitochondria Nucleus, Mitochondria 

Staining characteristics

  

Staining intensity

Weak Moderate 

Validation

Multiple locations partly supported (at least one) by experimental gene/protein characterization data.
 
Multiple locations partly supported (at least one) by experimental gene/protein characterization data.
 

Comment

  

         Cell line U-251 MG

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Location(s)

Cytoplasm Nucleus, Mitochondria 

Staining characteristics

  

Staining intensity

Moderate Weak 

Validation

Location not consistent with experimental gene/protein characterization data.
 
Multiple locations partly supported (at least one) by experimental gene/protein characterization data.
 

Comment

  

         Antibody summary


Immunofluorescent staining of human cell line U-2 OS shows positivity in cytoplasm & mitochondria.
 

Immunofluorescent staining of human cell line A-431 shows positivity in nucleus but not nucleoli & mitochondria.
 

Summary

Staining of cytoplasm in all three cell lines with additional staining of mitochondria in U-2 OS.   Staining of mitochondria and nucleus in all three cell lines.  

Validation

The subcellular location is partly supported by literature or no literature is available.
 
The subcellular location is supported by literature.
 

Dictionary

Cytoplasm
Mitochondria
Nucleus but not nucleoli