Fields »

SUBCELLULAR LOCATION

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Main location(s)

Cytoplasm

Additional location(s)

Nucleus 

Staining summary

Mainly localized to cytoplasm. In addition localized to nucleus.

Reliability (APE)

Medium

Antibodies in assay

CAB003852, HPA007981
 

Antibody CAB003852

 

Antibody HPA007981

 

         Cell line A-431

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Location(s)

 Cytoplasm 

Staining characteristics

 Granular 

Staining intensity

Negative Moderate 

Validation

No staining.
 
One/multiple location(s) supported by experimental gene/protein characterization data.
 

Comment

  

         Cell line U-2 OS

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Location(s)

 Cytoplasm 

Staining characteristics

 Granular 

Staining intensity

Negative Weak 

Validation

No staining.
 
One/multiple location(s) supported by experimental gene/protein characterization data.
 

Comment

  

         Cell line U-251 MG

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Location(s)

 Nucleus, Cytoplasm 

Staining characteristics

 Granular 

Staining intensity

Negative Weak 

Validation

No staining.
 
One/multiple location(s) supported by experimental gene/protein characterization data.
 

Comment

  

         Antibody summary


Immunofluorescent staining of human cell line U-2 OS shows no positivity.
 

Immunofluorescent staining of human cell line A-431 shows positivity in cytoplasm.
 

Summary

No staining was observed in any of the three cell lines.   Staining of cytoplasm in all three cell lines with additional staining of nucleus in U-251MG.  

Validation

The subcellular location is partly supported by literature or no literature is available.
 
The subcellular location is supported by literature.
 

Dictionary

Cytoplasm
Nucleus