BCL11A

ANTIBODY AND ANTIGEN INFORMATION

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Antibody HPA029003

 

Antibody CAB014891

 

ANTIBODY INFORMATION

Provider

Atlas Antibodies
Sigma-Aldrich
 SDIX
 

Product name

HPA029003 2125 

Host species

Rabbit Rabbit 

Clonality

pAb msAb 

Purity

Affinity purified using the PrEST-antigen as affinity ligand Affinity 

Released in version

12 4 

ANTIGEN INFORMATION

Antigen

Recombinant protein fragment Genetic immunization 

Length (aa)

78  

Antigen sequence

LSTASSPEPGTSDLVGSASSALKSVVAKFKSENDPNLIPENGDEEEEEDD
EEEEEEEEEEEEELTESERVDYGFGLSL
 
 

Matching transcripts

BCL11A-001 - ENSP00000338774 [100%]
BCL11A-002 - ENSP00000349300 [100%]
BCL11A-006 - ENSP00000351307 [100%]
BCL11A-201 - ENSP00000443712 [100%]
BCL11A-202 - ENSP00000438303 [100%]
  

Other gene match

  

ANTIBODY VALIDATION

         Immunohistochemistry

Image

  

Description

 Immunohistochemical staining of human gall bladder shows cytoplasmic and nuclear positivity in glandular cells.
More information
 

Retrieval method

 HIER pH6 

Antibody dilution

 1:1000 

Validation IH

 Uncertain: Two (or more) antibodies yielding partly similar staining patterns which are partly consistent with gene/protein characterization data or consistent with limited gene/protein characterization data 

         Immunofluorescence

Image

  

Description

Immunofluorescent staining of human cell line U-2 OS shows positivity in nucleus but not nucleoli.
More information
 Immunofluorescent staining of human cell line U-2 OS shows positivity in nucleus but not nucleoli.
More information
 

Antibody dilution

1:76 1:112 

Validation IF

Supportive: The subcellular location is supported by literature. Supportive: The subcellular location is supported by literature. 

         Western Blot

Image

  

Description

  

Target mass (kDa)

91.2, 87.6, 86.4, 83.9, 47.9 91.2, 87.6, 86.4, 83.9, 47.9, 26.9 

Antibody dilution

1:250 1:500 

Validation WB

Non-supportive: Weak band of predicted size but with additional bands of higher intensity also present Non-supportive: Only bands not corresponding to the predicted size 

         Protein array

Image

  

Description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
More information
  

Antibody dilution

1:3000  

Validation PA

Uncertain: Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).  
 

ANTIGEN VIEW

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BCL11A-001
 
BCL11A-002
 
BCL11A-006
 
BCL11A-201
 
BCL11A-202