Fields »

SUBCELLULAR LOCATION

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Main location(s)

Nucleus but not nucleoli

Additional location(s)

Cytoplasm, Vesicles 

Staining summary

Mainly localized to the nucleus. In addition localized to vesicles and cytoplasm.

Reliability (APE)

Low

Antibodies in assay

CAB004851, HPA027545
 

Antibody CAB004851

 

Antibody HPA027545

 

         Cell line A-431

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Location(s)

 Nucleus but not nucleoli, Vesicles 

Staining characteristics

 Granular 

Staining intensity

Negative Moderate 

Validation

No staining.
 
Not decisive - One/multiple location(s) where experimental gene/protein characterization data is partly supporting and partly conflicting.
 

Comment

  

         Cell line U-2 OS

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Location(s)

 Nucleus but not nucleoli, Vesicles 

Staining characteristics

 Granular 

Staining intensity

Negative Strong 

Validation

No staining.
 
Not decisive - One/multiple location(s) where experimental gene/protein characterization data is partly supporting and partly conflicting.
 

Comment

  

         Cell line U-251 MG

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Location(s)

 Nucleus but not nucleoli, Cytoplasm 

Staining characteristics

  

Staining intensity

Negative Moderate 

Validation

No staining.
 
Not decisive - One/multiple location(s) where experimental gene/protein characterization data is partly supporting and partly conflicting.
 

Comment

  

         Antibody summary


Immunofluorescent staining of human cell line A-431 shows no positivity.
 

Immunofluorescent staining of human cell line U-2 OS shows positivity in nucleus but not nucleoli & vesicles.
 

Summary

No staining was observed in any of the three cell lines.   Staining of nucleus in all three cell lines. Additional staining of cytoplasm in U-251 MG and vesicles in A-431 and U-2 OS.  

Validation

The subcellular location is partly supported by literature or no literature is available.
 
The subcellular location is partly supported by literature or no literature is available.
 

Dictionary

Cytoplasm
Nucleus but not nucleoli
Endosome