Fields »

SUBCELLULAR LOCATION

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Main location(s)

Nucleus but not nucleoli, Vesicles

Additional location(s)

Cytoplasm 

Staining summary

Localized to vesicles or to the nucleus and cytoplasm.

Reliability (APE)

Very low

Antibodies in assay

HPA024123, HPA027539
 

Antibody HPA024123

 

Antibody HPA027539

 

         Cell line A-431

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Location(s)

Vesicles Nucleus but not nucleoli, Cytoplasm 

Staining characteristics

  

Staining intensity

Strong Weak 

Validation

The multi-targeting antibody yielding a staining pattern with no available gene/protein characterization data.
 
One/multiple location(s) with no available experimental gene/protein characterization data or partly supported and partly conflicted, but showing dissimilar staining to ≥1 other antibody.
 

Comment

  

         Cell line U-2 OS

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Location(s)

Vesicles Nucleus but not nucleoli, Cytoplasm 

Staining characteristics

  

Staining intensity

Moderate Weak 

Validation

The multi-targeting antibody yielding a staining pattern with no available gene/protein characterization data.
 
One/multiple location(s) with no available experimental gene/protein characterization data or partly supported and partly conflicted, but showing dissimilar staining to ≥1 other antibody.
 

Comment

  

         Cell line U-251 MG

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Location(s)

Vesicles   

Staining characteristics

   

Staining intensity

Moderate   

Validation

The multi-targeting antibody yielding a staining pattern with no available gene/protein characterization data.
   

Comment

   

         Antibody summary


Immunofluorescent staining of human cell line U-2 OS shows positivity in vesicles.
 

Immunofluorescent staining of human cell line U-2 OS shows positivity in nucleus but not nucleoli & cytoplasm.
 

Summary

Staining of vesicles in all three cell lines.   Staining of nucleus and cytoplasm in A-431 and U-2 OS.  

Validation

The subcellular location is partly supported by literature or no literature is available.
 
The subcellular location is partly supported by literature or no literature is available.
 

Dictionary

Cytoplasm
Nucleus but not nucleoli
Endosome