Fields »

SUBCELLULAR LOCATION

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Main location(s)

Nucleus

Additional location(s)

Nucleoli, Cytoskeleton (Intermediate filaments) 

Staining summary

Staining of nucleus in all three cell lines, with additional staining of nuceloli and cytoskeleton (intermediate filaments) in HeLa and PC-3.

Reliability (Single)

Antibodies in assay

HPA051446
 

Antibody HPA051446

 

         Cell line HeLa

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Location(s)

Nucleus, Nucleoli, Cytoskeleton (Intermediate filaments) 

Staining characteristics

Spotty 

Staining intensity

Weak 

Validation

One/multiple location(s) with no available experimental gene/protein characterization data.
 

Comment

 

         Cell line PC-3

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Location(s)

Nucleus, Nucleoli, Cytoskeleton (Intermediate filaments) 

Staining characteristics

Spotty 

Staining intensity

Strong 

Validation

One/multiple location(s) with no available experimental gene/protein characterization data.
 

Comment

 

         Cell line U-2 OS

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Location(s)

Nucleus 

Staining characteristics

Spotty 

Staining intensity

Weak 

Validation

One/multiple location(s) with no available experimental gene/protein characterization data.
 

Comment

 

         Antibody summary


Immunofluorescent staining of human cell line HeLa shows positivity in nucleus, nucleoli & cytoskeleton (intermediate filaments).
 

Summary

Staining of nucleus in all three cell lines, with additional staining of nuceloli and cytoskeleton (intermediate filaments) in HeLa and PC-3.  

Validation

The subcellular location is partly supported by literature or no literature is available.
 

Dictionary

Intermediate filaments
Nucleoli
Nucleus