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SUBCELLULAR LOCATION

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Main location(s)

Plasma membrane, Cytoplasm

Additional location(s)

Vesicles 

Staining summary

Mainly localized to the plasma membrane and cytoplasm. In addition localized to vesicles.

Reliability (APE)

Medium

Antibodies in assay

HPA019010, HPA019070, HPA026643
 

Antibody HPA019010

 

Antibody HPA019070

 

Antibody HPA026643

 

         Cell line A-431

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Location(s)

Plasma membrane, Cytoplasm Plasma membrane, Cytoplasm Plasma membrane, Cytoplasm, Vesicles 

Staining characteristics

   

Staining intensity

Moderate Strong Strong 

Validation

Location not consistent with experimental gene/protein characterization data, but supported by ≥1 other antibody.
 
Location not consistent with experimental gene/protein characterization data, but supported by ≥1 other antibody.
 
Location not consistent with experimental gene/protein characterization data.
 

Comment

   

         Cell line U-2 OS

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Location(s)

Plasma membrane, Cytoplasm Plasma membrane, Cytoplasm Plasma membrane, Cytoplasm, Vesicles 

Staining characteristics

   

Staining intensity

Moderate Strong Strong 

Validation

Location not consistent with experimental gene/protein characterization data, but supported by ≥1 other antibody.
 
Location not consistent with experimental gene/protein characterization data, but supported by ≥1 other antibody.
 
Location not consistent with experimental gene/protein characterization data.
 

Comment

   

         Cell line U-251 MG

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Location(s)

Plasma membrane, Cytoplasm Plasma membrane, Cytoplasm Plasma membrane, Cytoplasm, Vesicles 

Staining characteristics

 Smooth  

Staining intensity

Moderate Strong Moderate 

Validation

Location not consistent with experimental gene/protein characterization data, but supported by ≥1 other antibody.
 
Location not consistent with experimental gene/protein characterization data, but supported by ≥1 other antibody.
 
Location not consistent with experimental gene/protein characterization data.
 

Comment

   

         Antibody summary


Immunofluorescent staining of human cell line U-2 OS shows positivity in plasma membrane & cytoplasm.
 

Immunofluorescent staining of human cell line A-431 shows positivity in plasma membrane & cytoplasm.
 

Immunofluorescent staining of human cell line U-2 OS shows positivity in plasma membrane, cytoplasm & vesicles.
 

Summary

Staining of plasma membrane/cytoplasm in all three cell lines.   Staining of plasma membrane and cytoplasm in all three cell lines.   Staining of plasma membrane, cytoplasm and vesicles in all three cell lines.  

Validation

The subcellular location is partly supported by literature or no literature is available.
 
The subcellular location is partly supported by literature or no literature is available.
 
The subcellular location is not consistent with literature.
 

Dictionary

Cytoplasm
Plasma membrane
Endosome