Fields »

SUBCELLULAR LOCATION

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Main location(s)

Centrosome

Additional location(s)

Nucleus but not nucleoli, Cytoplasm 

Staining summary

Staining of centrosome in all three cell lines with additional staining of cytoplasm in A-431 and nucleus and cytoplasm in U-251 MG.

Reliability (Single)

Antibodies in assay

HPA020607
 

Antibody HPA020607

 

         Cell line A-431

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Location(s)

Cytoplasm, Centrosome 

Staining characteristics

 

Staining intensity

Weak 

Validation

One/multiple location(s) with no available experimental gene/protein characterization data.
 

Comment

 

         Cell line U-2 OS

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Location(s)

Centrosome 

Staining characteristics

 

Staining intensity

Weak 

Validation

One/multiple location(s) with no available experimental gene/protein characterization data.
 

Comment

 

         Cell line U-251 MG

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Location(s)

Nucleus but not nucleoli, Cytoplasm, Centrosome 

Staining characteristics

Granular 

Staining intensity

Moderate 

Validation

One/multiple location(s) with no available experimental gene/protein characterization data.
 

Comment

 

         Antibody summary


Immunofluorescent staining of human cell line A-431 shows positivity in cytoplasm & centrosome.
 

Summary

Staining of centrosome in all three cell lines with additional staining of cytoplasm in A-431 and nucleus and cytoplasm in U-251 MG.  

Validation

The subcellular location is partly supported by literature or no literature is available.
 

Dictionary

Centrosome
Cytoplasm
Nucleus but not nucleoli