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SUBCELLULAR LOCATION

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Main location(s)

Plasma membrane, Cytoplasm, Cytoskeleton (Actin filaments)

Additional location(s)

Golgi apparatus 

Staining summary

Staining of plasma membrane, cytoskeleton (actin filaments) and cytoplasm in all three cell lines. Additional staining of Golgi in A-431 and U-2 OS.

Reliability (Single)

Antibodies in assay

HPA006379
 

Antibody HPA006379

 

         Cell line A-431

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Location(s)

Plasma membrane, Cytoplasm, Golgi apparatus, Cytoskeleton (Actin filaments) 

Staining characteristics

 

Staining intensity

Moderate 

Validation

One/multiple location(s) supported by experimental gene/protein characterization data.
 

Comment

 

         Cell line U-2 OS

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Location(s)

Plasma membrane, Cytoplasm, Golgi apparatus, Cytoskeleton (Actin filaments) 

Staining characteristics

 

Staining intensity

Moderate 

Validation

One/multiple location(s) supported by experimental gene/protein characterization data.
 

Comment

 

         Cell line U-251 MG

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Location(s)

Plasma membrane, Cytoplasm, Cytoskeleton (Actin filaments) 

Staining characteristics

Granular 

Staining intensity

Moderate 

Validation

One/multiple location(s) supported by experimental gene/protein characterization data.
 

Comment

 

         Antibody summary


Immunofluorescent staining of human cell line U-2 OS shows positivity in plasma membrane, cytoplasm, golgi apparatus & cytoskeleton (actin filaments).
 

Summary

Staining of plasma membrane, cytoskeleton (actin filaments) and cytoplasm in all three cell lines. Additional staining of Golgi in A-431 and U-2 OS.  

Validation

The subcellular location is supported by literature.
 

Dictionary

Cytoplasm
Actin filaments
Golgi apparatus
Plasma membrane