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SUBCELLULAR LOCATION

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Main location(s)

Plasma membrane, Cell Junctions

Additional location(s)

Nucleus but not nucleoli 

Staining summary

Mainly localized to the plasma membrane and cell junctions. In addition localized to the nucleus.

Reliability (APE)

High

Antibodies in assay

CAB013496, HPA030212, HPA030213, HPA030215
 

Antibody CAB013496

 

Antibody HPA030212

 

Antibody HPA030213

 

Antibody HPA030215

 

         Cell line A-431

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Location(s)

Nucleus but not nucleoli, Cytoplasm, Cell Junctions Nucleus but not nucleoli, Plasma membrane, Cell Junctions Nucleus but not nucleoli, Plasma membrane, Cell Junctions Plasma membrane, Cytoplasm, Cell Junctions 

Staining characteristics

Granular    

Staining intensity

Moderate Moderate Moderate Weak 

Validation

Multiple locations partly supported (at least one) by experimental gene/protein characterization data.
 
One/multiple location(s) supported by experimental gene/protein characterization data and ≥1 other antibody.
 
One/multiple location(s) supported by experimental gene/protein characterization data and ≥1 other antibody.
 
Multiple locations partly supported (at least one) by experimental gene/protein characterization data.
 

Comment

    

         Cell line U-2 OS

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Location(s)

Nucleus but not nucleoli, Cytoplasm, Cell Junctions Nucleus but not nucleoli, Plasma membrane, Cell Junctions Nucleus but not nucleoli, Plasma membrane, Cell Junctions Cytoplasm, Cell Junctions 

Staining characteristics

Granular  Speckle  

Staining intensity

Weak Strong Strong Moderate 

Validation

Multiple locations partly supported (at least one) by experimental gene/protein characterization data.
 
One/multiple location(s) supported by experimental gene/protein characterization data and ≥1 other antibody.
 
One/multiple location(s) supported by experimental gene/protein characterization data and ≥1 other antibody.
 
Multiple locations partly supported (at least one) by experimental gene/protein characterization data.
 

Comment

    

         Cell line U-251 MG

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Location(s)

Cytoplasm Nucleus but not nucleoli, Cell Junctions Plasma membrane, Cell Junctions Cytoplasm, Cell Junctions 

Staining characteristics

Granular    

Staining intensity

Moderate Strong Strong Moderate 

Validation

Location not consistent with experimental gene/protein characterization data.
 
One/multiple location(s) supported by experimental gene/protein characterization data and ≥1 other antibody.
 
One/multiple location(s) supported by experimental gene/protein characterization data and ≥1 other antibody.
 
Multiple locations partly supported (at least one) by experimental gene/protein characterization data.
 

Comment

    

         Antibody summary


Immunofluorescent staining of human cell line U-2 OS shows positivity in nucleus but not nucleoli, cytoplasm & cell junctions.
 

Immunofluorescent staining of human cell line A-431 shows positivity in nucleus but not nucleoli, plasma membrane & cell junctions.
 

Immunofluorescent staining of human cell line U-251MG shows positivity in plasma membrane & cell junctions.
 

Immunofluorescent staining of human cell line U-251 MG shows positivity in cytoplasm & cell junctions.
 

Summary

Staining of cytoplasm in all three cell lines with additional staining of nucleus and cell junctions in A-431 and U-2 OS.   Staining of cell junctions, plasma membrane and nucleus in all three cell lines.   Staining of cell junctions, plasma membrane and nucleus in all three cell lines.   Staining of cell junctions and cytoplasm in all three cell lines. Additional staining of plasma membrane in A-431.  

Validation

The subcellular location is supported by literature.
 
The subcellular location is supported by literature.
 
The subcellular location is supported by literature.
 
The subcellular location is supported by literature.
 

Dictionary

Cell junctions
Nucleus but not nucleoli
Plasma membrane