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SUBCELLULAR LOCATION

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Main location(s)

Nucleus, Plasma membrane, Cytoplasm

Additional location(s)

 

Staining summary

Localized to the plasma membrane, cytoplasm and nucleus.

Reliability (APE)

Low

Antibodies in assay

HPA028399, HPA028652
 

Antibody HPA028399

 

Antibody HPA028652

 

         Cell line A-431

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Location(s)

Nucleus but not nucleoli, Plasma membrane, Cytoplasm Nucleus, Cytoplasm 

Staining characteristics

 Speckle 

Staining intensity

Moderate Moderate 

Validation

Location not consistent with experimental gene/protein characterization data.
 
Location not consistent with experimental gene/protein characterization data.
 

Comment

  

         Cell line U-2 OS

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Location(s)

Nucleus but not nucleoli, Plasma membrane, Cytoplasm Nucleus but not nucleoli, Cytoplasm 

Staining characteristics

Spotty Speckle 

Staining intensity

Strong Weak 

Validation

Location not consistent with experimental gene/protein characterization data.
 
Location not consistent with experimental gene/protein characterization data.
 

Comment

  

         Cell line U-251 MG

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Location(s)

Nucleus but not nucleoli, Plasma membrane, Cytoplasm Nucleus but not nucleoli, Cytoplasm 

Staining characteristics

 Speckle 

Staining intensity

Moderate Moderate 

Validation

Location not consistent with experimental gene/protein characterization data.
 
Location not consistent with experimental gene/protein characterization data.
 

Comment

  

         Antibody summary


Immunofluorescent staining of human cell line A-431 shows positivity in nucleus but not nucleoli, plasma membrane & cytoplasm.
 

Immunofluorescent staining of human cell line U-2 OS shows positivity in nucleus but not nucleoli & cytoplasm.
 

Summary

Staining of nucleus, cytoplasm and plasma membrane in all three cell lines.   Staining of nucleus and cytoplasm in all three cell lines.  

Validation

The subcellular location is not consistent with literature.
 
The subcellular location is not consistent with literature.
 

Dictionary

Cytoplasm
Nucleus
Plasma membrane