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SUBCELLULAR LOCATION

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Main location(s)

Cytoplasm

Additional location(s)

 

Staining summary

Localized to the cytoplasm.

Reliability (APE)

High

Antibodies in assay

HPA017936, HPA018950, HPA018954
 

Antibody HPA017936

 

Antibody HPA018950

 

Antibody HPA018954

 

         Cell line A-431

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Location(s)

  Nucleus, Cytoplasm Cytoplasm 

Staining characteristics

  Spotty  

Staining intensity

  Moderate Moderate 

Validation

  
Multiple locations partly supported (at least one) by experimental gene/protein characterization data.
 
One/multiple location(s) supported by experimental gene/protein characterization data and ≥1 other antibody.
 

Comment

    

         Cell line U-2 OS

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Location(s)

  Nucleus, Cytoplasm Cytoplasm 

Staining characteristics

  Spotty  

Staining intensity

  Moderate Moderate 

Validation

  
Multiple locations partly supported (at least one) by experimental gene/protein characterization data.
 
One/multiple location(s) supported by experimental gene/protein characterization data and ≥1 other antibody.
 

Comment

    

         Cell line U-251 MG

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Location(s)

Cytoplasm Nucleus, Cytoplasm Cytoplasm 

Staining characteristics

 Spotty  

Staining intensity

Moderate Moderate Moderate 

Validation

One/multiple location(s) supported by experimental gene/protein characterization data and ≥1 other antibody.
 
Multiple locations partly supported (at least one) by experimental gene/protein characterization data.
 
One/multiple location(s) supported by experimental gene/protein characterization data and ≥1 other antibody.
 

Comment

   

         Antibody summary


Immunofluorescent staining of human cell line U-251MG shows positivity in cytoplasm.
 

Immunofluorescent staining of human cell line U-251MG shows positivity in nucleus & cytoplasm.
 

Immunofluorescent staining of human cell line U-2 OS shows positivity in cytoplasm.
 

Summary

Staining of cytoplasm in U-251 MG.   Staining of cytoplasm and spots in nucleus in all three cell lines.   Staining of cytoplasm in all three cell lines.  

Validation

The subcellular location is supported by literature.
 
The subcellular location is supported by literature.
 
The subcellular location is supported by literature.
 

Dictionary

Cytoplasm