CTSL protein expression summary - The Human Protein Atlas

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CTSL

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HUMAN PROTEIN ATLAS SUMMARYⁱ General description of the gene and encoded protein(s) using information from HGNC and Ensembl, as well as predictions made as well as predictions made by the Human Protein Atlas project.
Protein	Cathepsin L
Gene nameⁱ Gene name according to HGNC.	CTSL (CTSL1, FLJ31037)
Tissue specificityⁱ The RNA specificity category is based on mRNA expression levels in the analyzed samples based on data from HPA. The categories include: cell type enriched, group enriched, cell type enhanced, low cell type specificity and not detected. Read more	Tissue enhanced (placenta)
Subcellular locationⁱ The main location is characterized by presence in all tested cell lines and/or increased intensity compared to other locations. Additional locations are characterized by either a markedly lower staining intensity than the main location, or that it is only observed in a subset of the cell lines.	Golgi apparatus and additionally in Vesicles
Cancer prognostic summary	Prognostic marker in lung cancer (unfavourable), glioma (unfavourable) and renal cancer (favourable)
Brain specificityⁱ The RNA specificity category is based on mRNA expression levels in the analyzed samples based on data from HPA. The categories include: cell type enriched, group enriched, cell type enhanced, low cell type specificity and not detected. Read more	Low human brain regional specificity
Blood specificityⁱ The RNA specificity category is based on mRNA expression levels in the analyzed samples based on data from HPA. The categories include: cell type enriched, group enriched, cell type enhanced, low cell type specificity and not detected. Read more	Group enriched (non-classical monocyte, intermediate monocyte)
General main location (Predicted)ⁱ All transcripts of all genes have been analyzed regarding the location(s) of corresponding protein based on prediction methods for signal peptides and transmembrane regions. Genes with at least one transcript predicted to encode a secreted protein, according to prediction methods or to UniProt location data, have been further annotated and classified with the aim to determine if the corresponding protein(s) are secreted or actually retained in intracellular locations or membrane-attached. Remaining genes, with no transcript predicted to encode a secreted protein, will be assigned the prediction-based location(s). The annotated location overrules the predicted location, so that a gene encoding a predicted secreted protein that has been annotated as intracellular will have intracellular as the final location. Read more	Intracellular
Protein function (UniProt)ⁱ Useful information about the protein provided by UniProt.	Important for the overall degradation of proteins in lysosomes.... show less
Molecular function (UniProt)ⁱ Keywords assigned by UniProt to proteins due to their particular molecular function.	Hydrolase, Protease, Thiol protease
Gene summary (Entrez)ⁱ Useful information about the gene from Entrez	The protein encoded by this gene is a lysosomal cysteine proteinase that plays a major role in intracellular protein catabolism. Its substrates include collagen and elastin, as well as alpha-1 protease inhibitor, a major controlling element of neutrophil elastase activity. The encoded protein has been implicated in several pathologic processes, including myofibril necrosis in myopathies and in myocardial ischemia, and in the renal tubular response to proteinuria. This protein, which is a member of the peptidase C1 family, is a dimer composed of disulfide-linked heavy and light chains, both produced from a single protein precursor. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Apr 2012]... show less

TISSUE ATLASⁱ The Tissue Atlas contains information regarding the expression profiles of human genes both on the mRNA and protein level. The protein expression data from 44 normal human tissue types is derived from antibody-based protein profiling using immunohistochemistry. Read more

Tissue specificity (RNA)ⁱ The RNA specificity category is based on mRNA expression levels in the analyzed samples based on a combination of data from HPA, GTEX and FANTOM5. The categories include: tissue enriched, group enriched, tissue enhanced, low tissue specificity and not detected. Read more	Tissue enhanced (placenta)
Tissue distribution (RNA)ⁱ The RNA distribution category is based on mRNA expression levels in the analyzed samples based on a combination of data from HPA, GTEX and FANTOM5. The categories include: detected in all, detected in many, detected in some, detected in single and not detected. Read more	Detected in all
Protein expressionⁱ A summary of the overall protein expression pattern across the analyzed normal tissues. The summary is based on knowledge-based annotation. "Estimation of protein expression could not be performed. View primary data." is shown for genes analyzed with a knowledge-based approach where available RNA-seq and gene/protein characterization data has been evaluated as not sufficient in combination with immunohistochemistry data to yield a reliable estimation of the protein expression profile. Read more	Lysosomal expression in several tissues.

Human regional specificityⁱ

Low region specificity

CELL ATLASⁱ Summary of data presented in the Cell Atlas and a representative image of subcellular localization. The Cell Atlas provides RNA expression data derived from RNA sequencing of a large panel of cell lines and protein localization data derived from antibody-based profiling by immunofluorescence confocal microscopy, using a subset of cell lines selected based on RNA expression. Read more

Main locationⁱ The main location is characterized by presence in all tested cell lines and/or increased intensity compared to other locations. It is highlighted in the illustration to the right. If available, links to overrepresentation analyses in Reactome, a free, open-source, curated and peer reviewed biological pathway database, are provided. An analysis is done for the corresponding gene set of the proteome localizing to the main and additional locations of the protein on this page, respectively.	Localized to the Golgi apparatus
Additional locationⁱ Additional locations are characterized by either a markedly lower staining intensity than the main location, or that it is only observed in a subset of the cell lines. They are highlighted in the illustration to the right. If available, links to overrepresentation analyses in Reactome, a free, open-source, curated and peer reviewed biological pathway database, are provided. An analysis is done for the corresponding gene set of the proteome localizing to the main and additional locations of the protein on this page, respectively.	In addition localized to the Vesicles
Cell line specificityⁱ The cell lines in the Human Protein Atlas have been analyzed by RNA-seq to estimate the transcript abundance of each protein-coding gene. The RNA-seq data was then used to classify all genes according to their cell line-specific expression into one of six different categories, defined based on the total set of all NX values in all analyzed cell lines. Read more	Cell line enhanced (ASC TERT1, HSkMC)

BLOOD ATLASⁱ The Blood Atlas contains single cell type information on genome-wide RNA expression profiles of human protein-coding genes covering various B- and T-cells, monocytes, granulocytes and dendritic cells. In addition, an analysis of the human secretome is presented. including annotation of the genes predicted to be actively secreted to human blood. An analysis of the proteins detected in human blood is also presented. Read more

Secretome annotationⁱ All genes with at least one predicted secreted isoform have been annotated and classified with the aim to determine if the corresponding protein(s) are: secreted into blood locally secreted or actually being attached to membrane or retained in intracellular locations like mitochondria, endoplasmatic reticulum (ER), Golgi apparatus or lysosomes. A gene will only have one location and the categories and their hierarchy is: Intracellular and membrane Secreted to blood Secreted in brain Secreted to digestive system Secreted in female reproductive system Secreted in male reproductive system Secreted to extracellular matrix Secreted in other tissues Secreted - unknown location Read more	Intracellular and membrane
Immune cell specificityⁱ The RNA specificity category is based on mRNA expression levels in the analyzed samples based on data from HPA. The categories include: cell type enriched, group enriched, cell type enhanced, low cell type specificity and not detected. Read more	Group enriched (non-classical monocyte, intermediate monocyte)
Detected in blood by immunoassayⁱ The blood-based immunoassay category applies to actively secreted proteins and is based on plasma or serum protein concentrations established with enzyme-linked immunosorbent assays, compiled from a literature search. The categories include: detected and not detected, where detection refers to a concentration found in the literature search. Read more	No (not applicable)
Detected in blood by mass spectrometryⁱ Detection or not of the gene in blood, based on spectral count estimations from a publicly available mass spectrometry-based plasma proteomics data set obtained from the PeptideAtlas.	Yes

PATHOLOGY ATLASⁱ

Lung cancer p<0.001

Prognostic summary	Prognostic marker in lung cancer (unfavourable), glioma (unfavourable) and renal cancer (favourable)
Cancer specificity (RNA)ⁱ Specificity of RNA expression in 17 cancer types is categorized as either cancer enriched, group enriched, cancer enhanced, low cancer specificity and not detected. Read more	Low cancer specificity
Cancer distribution (RNA)ⁱ Distribution of RNA expression in 17 cancer types is categorized as either detected in single, detected in some, detected in many, detected in all, or not detected. Read more	Detected in all

GENE INFORMATIONⁱ Gene information from Ensembl and Entrez, as well as links to available gene identifiers are displayed here. Information was retrieved from Ensembl if not indicated otherwise.
Gene name	CTSL (HGNC Symbol)
Synonyms	CTSL1, FLJ31037
Description	Cathepsin L (HGNC Symbol)
Chromosome	9
Cytoband	q21.33
Chromosome location (bp)	87725519 - 87731393
Number of transcriptsⁱ Number of protein-coding transcribed from this gene as defined by Ensembl.	3
Protein evidence	Evidence at protein level (all genes)
Protein classⁱ Protein class(es) of the gene product according to selected gene lists. List of protein classes.	Cancer-related genes Enzymes Plasma proteins
Ensembl	ENSG00000135047 (version 92.38)
Entrez gene	1514
UniProt	P07711 (UniProt - Evidence at protein level)
neXtProt	NX_P07711
Antibodypedia	CTSL antibodies

PROTEIN BROWSERⁱ

CTSL-201

CTSL-202

CTSL-203

PROTEIN INFORMATIONⁱ The protein information section displays alternative protein-coding transcripts (splice variants) encoded by this gene according to the Ensembl database. The ENSP identifier links to the Ensembl website protein summary, while the ENST identifier links to the Ensembl website transcript summary for the selected splice variant. The data in the UniProt column can be expanded to show links to all matching UniProt identifiers for this protein. The protein classes assigned to this protein are shown if expanding the data in the protein class column. Parent protein classes are in bold font and subclasses are listed under the parent class. The Gene Ontology terms assigned to this protein are listed if expanding the Gene ontology column. The length of the protein (amino acid residues according to Ensembl), molecular mass (kDalton), predicted signal peptide (according to a majority of the signal peptide predictors SPOCTOPUS, SignalP 4.0, and Phobius) and the number of predicted transmembrane region(s) (according to MDM) are also reported.
Splice variant	UniProt	Protein class	Gene ontology	Length & mass	Signal peptide (predicted)	Transmembrane regions (predicted)
CTSL-201 ENSP00000365061 ENST00000340342	P07711 [Direct mapping] Cathepsin L1 Cathepsin L1 heavy chain Cathepsin L1 light chain A0A024R276 [Target identity:100%; Query identity:100%] Cathepsin L, isoform CRA_a Show all	Enzymes ENZYME proteins Hydrolases Peptidases Cysteine-type peptidases MEMSAT3 predicted membrane proteins MEMSAT-SVM predicted membrane proteins Secreted proteins predicted by MDSEC SignalP predicted secreted proteins Phobius predicted secreted proteins SPOCTOPUS predicted secreted proteins Predicted intracellular proteins Plasma proteins Cancer-related genes Candidate cancer biomarkers Mapped to neXtProt neXtProt - Evidence at protein level Protein evidence (Peptideatlas) Show all	GO:0001968 [fibronectin binding] GO:0002224 [toll-like receptor signaling pathway] GO:0002250 [adaptive immune response] GO:0004197 [cysteine-type endopeptidase activity] GO:0004252 [serine-type endopeptidase activity] GO:0005515 [protein binding] GO:0005518 [collagen binding] GO:0005576 [extracellular region] GO:0005615 [extracellular space] GO:0005634 [nucleus] GO:0005764 [lysosome] GO:0006508 [proteolysis] GO:0008233 [peptidase activity] GO:0008234 [cysteine-type peptidase activity] GO:0016787 [hydrolase activity] GO:0019882 [antigen processing and presentation] GO:0019886 [antigen processing and presentation of exogenous peptide antigen via MHC class II] GO:0022617 [extracellular matrix disassembly] GO:0030574 [collagen catabolic process] GO:0036021 [endolysosome lumen] GO:0042393 [histone binding] GO:0043202 [lysosomal lumen] GO:0043394 [proteoglycan binding] GO:0045616 [regulation of keratinocyte differentiation] GO:0051603 [proteolysis involved in cellular protein catabolic process] GO:0070062 [extracellular exosome] GO:0071888 [macrophage apoptotic process] GO:0097067 [cellular response to thyroid hormone stimulus] GO:0097655 [serpin family protein binding] Show all	333 aa 37.6 kDa	Yes	0
CTSL-202 ENSP00000340470 ENST00000342020	Q5T8F0 [Direct mapping] Cathepsin L1 Show all	MEMSAT3 predicted membrane proteins MEMSAT-SVM predicted membrane proteins Secreted proteins predicted by MDSEC SignalP predicted secreted proteins Phobius predicted secreted proteins SPOCTOPUS predicted secreted proteins Predicted intracellular proteins Show all	GO:0006508 [proteolysis] GO:0008234 [cysteine-type peptidase activity] Show all	225 aa 25.5 kDa	Yes	0
CTSL-203 ENSP00000345344 ENST00000343150	P07711 [Direct mapping] Cathepsin L1 Cathepsin L1 heavy chain Cathepsin L1 light chain A0A024R276 [Target identity:100%; Query identity:100%] Cathepsin L, isoform CRA_a Show all	Enzymes ENZYME proteins Hydrolases Peptidases Cysteine-type peptidases MEMSAT3 predicted membrane proteins MEMSAT-SVM predicted membrane proteins Secreted proteins predicted by MDSEC SignalP predicted secreted proteins Phobius predicted secreted proteins SPOCTOPUS predicted secreted proteins Predicted intracellular proteins Plasma proteins Cancer-related genes Candidate cancer biomarkers Mapped to neXtProt neXtProt - Evidence at protein level Protein evidence (Peptideatlas) Show all	GO:0001968 [fibronectin binding] GO:0002224 [toll-like receptor signaling pathway] GO:0002250 [adaptive immune response] GO:0004197 [cysteine-type endopeptidase activity] GO:0004252 [serine-type endopeptidase activity] GO:0005515 [protein binding] GO:0005518 [collagen binding] GO:0005576 [extracellular region] GO:0005615 [extracellular space] GO:0005634 [nucleus] GO:0005764 [lysosome] GO:0006508 [proteolysis] GO:0008233 [peptidase activity] GO:0008234 [cysteine-type peptidase activity] GO:0016787 [hydrolase activity] GO:0019882 [antigen processing and presentation] GO:0019886 [antigen processing and presentation of exogenous peptide antigen via MHC class II] GO:0022617 [extracellular matrix disassembly] GO:0030574 [collagen catabolic process] GO:0036021 [endolysosome lumen] GO:0042393 [histone binding] GO:0043202 [lysosomal lumen] GO:0043394 [proteoglycan binding] GO:0045616 [regulation of keratinocyte differentiation] GO:0051603 [proteolysis involved in cellular protein catabolic process] GO:0070062 [extracellular exosome] GO:0071888 [macrophage apoptotic process] GO:0097067 [cellular response to thyroid hormone stimulus] GO:0097655 [serpin family protein binding] Show all	333 aa 37.6 kDa	Yes	0

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The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg Foundation.