Fields »

SUBCELLULAR LOCATION

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Main location(s)

Vesicles

Additional location(s)

Nucleus but not nucleoli 

Staining summary

Staining of vesicles in all three cell lines, with additional staining of nucleus in A-431 and U-251 MG.

Reliability (Single)

Antibodies in assay

CAB012185
 

Antibody CAB012185

 

         Cell line A-431

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Location(s)

Nucleus but not nucleoli, Vesicles 

Staining characteristics

 

Staining intensity

Strong 

Validation

Multiple locations partly supported (at least one) by experimental gene/protein characterization data.
 

Comment

 

         Cell line U-2 OS

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Location(s)

Vesicles 

Staining characteristics

 

Staining intensity

Weak 

Validation

One/multiple location(s) in cytoplasm (e.g. golgi, mitochondria) supported by experimental evidence for cytoplasmic localization.
 

Comment

 

         Cell line U-251 MG

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Location(s)

Nucleus but not nucleoli, Vesicles 

Staining characteristics

 

Staining intensity

Moderate 

Validation

Multiple locations partly supported (at least one) by experimental gene/protein characterization data.
 

Comment

 

         Antibody summary


Immunofluorescent staining of human cell line A-431 shows positivity in nucleus but not nucleoli & vesicles.
 

Summary

Staining of vesicles in all three cell lines, with additional staining of nucleus in A-431 and U-251 MG.  

Validation

The subcellular location is supported by literature.
 

Dictionary

Nucleus but not nucleoli
Endosome