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SUBCELLULAR LOCATION

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Main location(s)

Cytoplasm, Centrosome

Additional location(s)

Nucleus, Nucleoli 

Staining summary

Mainly localized to centrosome and cytoplasm but also nucleus and nucleoli.

Reliability (APE)

Low

Antibodies in assay

HPA008209, HPA023483
 

Antibody HPA008209

 

Antibody HPA023483

 

         Cell line A-431

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Location(s)

  Nucleus, Nucleoli, Cytoplasm, Centrosome 

Staining characteristics

  Fibrous 

Staining intensity

  Moderate 

Validation

  
Not decisive - One/multiple location(s) where experimental gene/protein characterization data is partly supporting and partly conflicting.
 

Comment

   

         Cell line U-2 OS

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Location(s)

 Nucleus, Cytoplasm, Centrosome 

Staining characteristics

 Fibrous 

Staining intensity

Negative Moderate 

Validation

No staining.
 
Not decisive - One/multiple location(s) where experimental gene/protein characterization data is partly supporting and partly conflicting.
 

Comment

  

         Cell line U-251 MG

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Location(s)

 Cytoplasm, Centrosome 

Staining characteristics

 Fibrous 

Staining intensity

Negative Moderate 

Validation

No staining.
 
Not decisive - One/multiple location(s) where experimental gene/protein characterization data is partly supporting and partly conflicting.
 

Comment

  

         Antibody summary


Immunofluorescent staining of human cell line U-251MG shows no positivity.
 

Immunofluorescent staining of human cell line U-251MG shows positivity in cytoplasm & centrosome.
 

Summary

No staining was observed in any of the three cell lines.   Staining of centrosome and cytoplasm in all three cell lines. Additional staining of nucleus in A-431 and U-2 OS.  

Validation

The subcellular location is partly supported by literature or no literature is available.
 
The subcellular location is partly supported by literature or no literature is available.
 

Dictionary

Centrosome
Cytoplasm
Nucleoli
Nucleus