Fields »

SUBCELLULAR LOCATION

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Main location(s)

Nucleus but not nucleoli, Cytoplasm

Additional location(s)

 

Staining summary

Localized to nucleus and cytoplasm.

Reliability (APE)

Medium

Antibodies in assay

CAB002685, HPA003056
 

Antibody CAB002685

 

Antibody HPA003056

 

         Cell line A-431

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Location(s)

  Nucleus but not nucleoli, Cytoplasm 

Staining characteristics

  Granular 

Staining intensity

  Moderate 

Validation

  
Multiple locations partly supported (at least one) by experimental gene/protein characterization data.
 

Comment

   

         Cell line U-2 OS

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Location(s)

Nucleus but not nucleoli, Cytoplasm Nucleus but not nucleoli 

Staining characteristics

 Speckle 

Staining intensity

Weak Strong 

Validation

Multiple locations partly supported (at least one) by experimental gene/protein characterization data.
 
Location not consistent with experimental gene/protein characterization data.
 

Comment

  

         Cell line U-251 MG

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Location(s)

Cytoplasm Nucleus but not nucleoli 

Staining characteristics

 Granular 

Staining intensity

Moderate Moderate 

Validation

One/multiple location(s) supported by experimental gene/protein characterization data.
 
Location not consistent with experimental gene/protein characterization data.
 

Comment

  

         Antibody summary


Immunofluorescent staining of human cell line U-251 MG shows positivity in cytoplasm.
 

Immunofluorescent staining of human cell line A-431 shows positivity in nucleus but not nucleoli & cytoplasm.
 

Summary

Staining of cytoplasm in U-251 MG and nucleus and cytoplasm in U-2 OS.   Staining of nucleus in all three cell lines with additional staining of cytoplasm in A-431.  

Validation

The subcellular location is supported by literature.
 
The subcellular location is partly supported by literature or no literature is available.
 

Dictionary

Cytoplasm
Nucleus but not nucleoli