HADH

ANTIBODY AND ANTIGEN INFORMATION

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Antibody HPA039588

 

Antibody HPA043888

 

ANTIBODY INFORMATION

Provider

Atlas Antibodies
Sigma-Aldrich
 Atlas Antibodies
Sigma-Aldrich
 

Product name

HPA039588 HPA043888 

Host species

Rabbit Rabbit 

Clonality

pAb pAb 

Purity

Affinity purified using the PrEST-antigen as affinity ligand Affinity purified using the PrEST-antigen as affinity ligand 

Released in version

7 12 

ANTIGEN INFORMATION

Antigen

Recombinant protein fragment Recombinant protein fragment 

Length (aa)

86 87 

Antigen sequence

YLMEAIRLYERGDASKEDIDTAMKLGAGYPMGPFELLDYVGLDTTKFIVD
GWHEMDAENPLHQPSPSLNKLVAENKFGKKTGEGFY
 
RLDKFAAEHTIFASNTSSLQITSIANATTRQDRFAGLHFFNPVPVMKLVE
VIKTPMTSQKTFESLVDFSKALGKHPVSCKDTPGFIV
 

Matching transcripts

HADH-001 - ENSP00000312288 [100%]
HADH-003 - ENSP00000425952 [100%]
HADH-201 - ENSP00000395167 [100%]
HADH-002 - ENSP00000385638 [83%]
HADH-202 - ENSP00000474560 [83%]
 HADH-001 - ENSP00000312288 [100%]
HADH-002 - ENSP00000385638 [100%]
HADH-003 - ENSP00000425952 [100%]
HADH-201 - ENSP00000395167 [100%]
HADH-202 - ENSP00000474560 [100%]
 

Other gene match

  

ANTIBODY VALIDATION

         Immunohistochemistry

Image

  

Description

Immunohistochemical staining of human liver shows strong cytoplasmic positivity in hepatocytes.
More information
  

Retrieval method

HIER pH6  

Antibody dilution

1:2000  

Validation IH

Supportive: One antibody yielding a staining pattern which is consistent with gene/protein characterization data  

         Immunofluorescence

Image

  

Description

Immunofluorescent staining of human cell line U-2 OS shows positivity in nucleus but not nucleoli & mitochondria.
More information
 Immunofluorescent staining of human cell line A-431 shows positivity in mitochondria & nucleus but excluded from the nucleoli.
More information
 

Antibody dilution

1:200 1:47 

Validation IF

Supportive: The subcellular location is supported by literature. Supportive: The subcellular location is supported by literature. 

         Western Blot

Image

  

Description

Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT-4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil
More information
 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT-4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil
More information
 

Target mass (kDa)

42.1, 36.1, 35.2, 34.3 42.1, 36.1, 35.2, 34.3 

Antibody dilution

1:250 1:250 

Validation WB

Supportive: Band of predicted size in kDa (+/-20%) with additional bands present Supportive: Band of predicted size in kDa (+/-20%) with additional bands present 

         Protein array

Image

  

Description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
More information
 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
More information
 

Antibody dilution

1:3000 1:12000 

Validation PA

Supportive: Pass with single peak corresponding to interaction only with its own antigen. Supportive: Pass with single peak corresponding to interaction only with its own antigen. 
 

ANTIGEN VIEW

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HADH-001
 
HADH-002
 
HADH-003
 
HADH-201
 
HADH-202