TUFT1

ANTIBODY AND ANTIGEN INFORMATION

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Antibody HPA028112

 

Antibody HPA054301

 

ANTIBODY INFORMATION

Provider

Atlas Antibodies
Sigma-Aldrich
 Atlas Antibodies
Sigma-Aldrich
 

Product name

HPA028112 HPA054301 

Host species

Rabbit Rabbit 

Clonality

pAb pAb 

Purity

Affinity purified using the PrEST-antigen as affinity ligand Affinity purified using the PrEST-antigen as affinity ligand 

Released in version

6 13 

ANTIGEN INFORMATION

Antigen

Recombinant protein fragment Recombinant protein fragment 

Length (aa)

99 80 

Antigen sequence

DCVAFEVTLSRYQREAEQSNVALQREEDRVEQKEAEVGELQRRLLGMETE
HQALLAKVREGEVALEELRSNNADCQAEREKAATLEKEVAGLREKIHHL
 
KSEVQYIQEARNCLQKLREDISSKLDRNLGDSLHRQEIQVVLEKPNGFSQ
SPTALYSSPPEVDTCINEDVESLRKTVQDL
 

Matching transcripts

TUFT1-006 - ENSP00000357842 [100%]
TUFT1-008 - ENSP00000357841 [100%]
TUFT1-010 - ENSP00000376476 [100%]
TUFT1-201 - ENSP00000343781 [100%]
TUFT1-202 - ENSP00000437997 [100%]
 TUFT1-006 - ENSP00000357842 [100%]
TUFT1-008 - ENSP00000357841 [100%]
TUFT1-201 - ENSP00000343781 [100%]
TUFT1-202 - ENSP00000437997 [100%]
 

Other gene match

  

ANTIBODY VALIDATION

         Immunohistochemistry

Image

  

Description

Immunohistochemical staining of human skin shows moderate positivity in a subset of keratinocytes.
More information
 Immunohistochemical staining of human kidney shows strong cytoplasmic positivity in cells in tubules.
More information
 

Retrieval method

HIER pH6 HIER pH6 

Antibody dilution

1:500 1:75 

Literature conformity

No avaliable characterization data No avaliable characterization data 

RNA consistency

Mainly not consistent with RNAseq data Mainly not consistent with RNAseq data 

         Western Blot

Image

  

Description

Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil
More information
 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil
More information
 

Target mass (kDa)

46.4, 44.3, 41.5, 38.0, 37.9 46.4, 44.3, 41.5, 37.9 

Antibody dilution

1:250 1:210 

Validation WB

Supportive: Band of predicted size in kDa (+/-20%) with additional bands present Uncertain: No bands detected 

         Protein array

Image

  

Description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
More information
 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
More information
 

Antibody dilution

1:3000 1:1650 

Validation PA

Supportive: Pass with single peak corresponding to interaction only with its own antigen. Supportive: Pass with single peak corresponding to interaction only with its own antigen. 

         Immunofluorescence

Image

  

Description

Application not done for this antibody. Immunofluorescent staining of human cell line A-431 shows positivity in vesicles.
More information
 

Antibody dilution

 1:41 

Validation IF

 Uncertain: The subcellular location is partly supported by literature or no literature is available. 
 

ANTIGEN VIEW

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TUFT1-006
 
TUFT1-008
 
TUFT1-010
 
TUFT1-201
 
TUFT1-202