The subcellular location page gives an overview of the subcellular location (in three cell lines) of protein(s) targeted by the antibody/antibodies for this gene. The main subcellular location of the encoded proteins and any additional locations are reported. Additional locations are characterized by either a markedly lower staining intensity than the main location, or that it was only observed in a subset of the cell lines. A short summary of the protein location is also given.
If the overview is based on one antibody only, the validation result for this antibody is reported by the "Reliability (Single)"-field (color coding described
here). If the overview is based on two or more antibodies, the reliability of the annotation is reported by the "Reliability (
APE)"-field (color-coding described
here). If the data is based on one automatically selected antibody (best IF score among available antibodies), the IF validation score for that antibody is reported as Reliability (Selected) (color-coding described
here).
The immunofluorescent staining using the antibody in each of the three different cell lines (A-431, U-2 OS and U-251 MG) is shown, together with annotated location, staining characteristics, staining intensity and
validation score. At the bottom of this page, a selected image for each antibody is shown, together with a summary of the staining in all studied cell lines and the validation score for the antibody. The assay is described
here.
Three different organelle probes are displayed as different channels in the multicolor images - nuclear stain in blue, micro-tubules in red and ER in yellow. The antibody staining is shown in green. By using the "toggle channels"-buttons, the different channels can be turned on and off.
All images are clickable for an enlarged view. The selected image will appear in large size and miniature images with all other staining results for this gene will be listed at the top left of the image. The selected image has a green overlay.
