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SUBCELLULAR LOCATION

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Main location(s)

Nucleus but not nucleoli, Plasma membrane

Additional location(s)

Cytoplasm, Cytoskeleton (Actin filaments) 

Staining summary

Staining of nucleus and plasma membrane in all three cell lines, with additional staining of cytoplasm in U-251 MG and cytoskeleton (actin filaments) in U-2 OS.

Reliability (Single)

Antibodies in assay

HPA050808
 

Antibody HPA050808

 

         Cell line A-549

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Location(s)

Nucleus but not nucleoli, Plasma membrane 

Staining characteristics

 

Staining intensity

Weak 

Validation

Multiple locations partly supported (at least one) by experimental gene/protein characterization data.
 

Comment

 

         Cell line U-2 OS

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Location(s)

Nucleus but not nucleoli, Plasma membrane, Cytoskeleton (Actin filaments) 

Staining characteristics

 

Staining intensity

Moderate 

Validation

Multiple locations partly supported (at least one) by experimental gene/protein characterization data.
 

Comment

 

         Cell line U-251 MG

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Location(s)

Nucleus but not nucleoli, Plasma membrane, Cytoplasm 

Staining characteristics

 

Staining intensity

Moderate 

Validation

Multiple locations partly supported (at least one) by experimental gene/protein characterization data.
 

Comment

 

         Antibody summary


Immunofluorescent staining of human cell line A-549 shows positivity in nucleus but not nucleoli & plasma membrane.
 

Summary

Staining of nucleus and plasma membrane in all three cell lines, with additional staining of cytoplasm in U-251 MG and cytoskeleton (actin filaments) in U-2 OS.  

Validation

The subcellular location is supported by literature.
 

Dictionary

Cytoplasm
Actin filaments
Nucleus but not nucleoli
Plasma membrane